Module 1.2- Phytochemical Tests (Alkaloids and Amine Oxide)) Flashcards
Reagent for Dragendorff’s test
Potassium bismuth iodide solution
1 mL extract + 1 mL reagent
Positive result for Dragendorff’s test
Orange-red precipitate for presence of alkaloids
Reagent for Mayer’s test
Potassium mercuric iodide solution
1 mL extract + 1 mL reagent
Positive result for Mayer’s test
Whitish or cream colored precipitate for presence of alkaloids
Reagent for Hager’s test
Saturated aqueous solution of picric acid
1 mL extract + 3 mL reagent
Positive result for Hager’s test
Yellow colored precipitate for presence of alkaloids
Reagent for Wagner’s test
Iodine in potassium iodide
1 mL extract + 2 mL reagent
Positive result for Wagner’s test
Reddish brown colored precipitate for presence of alkaloids
Reagent for Tannic acid test
Freshly prepared 5% saturated solution of tannic acid
1 mL extract + 1 mL reagent
Positive result for Tannic acid test
Precipitate
A small amount of caffeine is taken in porcelain dish + small amount of Potassium chlorate + one drop of HCl
- to dryness
- exposed to NH3 vapour
Murexide Test
Positive result for Murexide test
Purple color
Positive result for Mayer’s test for PRELIMINARY, CONFIRMATORY, QUATERNARY
BASES AND/OR AMINE OXIDE
(++) Definite turbidity
(+++) Heavy precipitation
*Yellowish or white ppt.
Positive result for Dragendorff’s
test for PRELIMINARY, CONFIRMATORY, QUATERNARY
BASES AND/OR AMINE OXIDE
(++) Definite turbidity
(+++) Heavy precipitation
*Orange-red ppt
Positive result for Wagner’s Test for PRELIMINARY, CONFIRMATORY, QUATERNARY
BASES AND/OR AMINE OXIDE
(++) Definite turbidity
(+++) Heavy precipitation
*Brown or reddish brown color ppt.
