Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Integumentary Sys > Module 1 MiniModule 6: Diagnostic Tests and Techniques > Flashcards

Module 1 MiniModule 6: Diagnostic Tests and Techniques Flashcards

1
Q

What are the types of scrapes?

A
  • Superficial
  • Deep
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

List the ways to collect skin cytology:

A
  • Tape
  • Surface
    - #10 Blade
    - Spatula
  • Cotton Swab
  • Direct Impression
  • Fine Needle Aspirate
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

List the types of cultures:

A
  • Bacterial
    • Aerobic
    • Anaerobic
    • Mycobacterial
  • Fungal
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

A surface mite that instead of residing in the hair follicles, dwells in the keratin layer of the epidermis, causing pruritic and contagious skin disease in cats

A

Demodex gatoi

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Screening – Superficial Skin Scrape (SSS)

List the Surface Mites:

A
  • Sarcoptes
  • Notoedres cati
  • Demodex gatoi
  • Cheyletiella
  • Otodectes cynotis
  • Trombicula
  • Lynxacarus radovsky
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

(T/F) For Superficial Skin Scrape is better if you cover a small area

A

False, cover a LARGE surface area
- generally fewer mites
- may need several slides to spread collected material
- perform at multiple sites

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

(T/F) If the superficial skin scrape is negative it does not R/O disease

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What type of Scrape is the following describing?
- Indication
- Follicular Parasites
- Demodex spp. (exception D. gatoi)
- Scrape small focal areas
- Pinch skin
- Induce capillary bleeding
- If negative usually RULES OUT disease
(Exception: hyperplastic tissue)

A

Deep Skin Scrape

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Cutaneous & Otic Cytology
In every derm case, what should you ask yourself?

A

“What infections are present?”

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Surface Cytology:
What are some transmissible diseases that may pass from animal to animal without changing the blade?

A
  • FIV, FELV, Papillomavirus
  • Rickettsia, Bartonella
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What is the following describing?
- #10 blade method
- NO mineral oil
- Smear material on glass slide
- Heat fix and stain
- Diff Quick/ Gram/ Methylene blue

A

Surface Cytology

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Surface Cytology:
What stain are you using for an Acetate Tape Prep?

A

Basophilic Stain
- Examine under oil immersion (100x objective)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

(T/F) A down side to using acetate tape prep is that it picks up TONS of debris which makes interpretation difficult but good for yeast

A

True

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

List the lesions for which Impression Smear would work best for:

A
  • Pustules
  • Erosions
  • Ulcers
  • Draining lesions
  • Under surface of crusts
  • Cut/ exposed surface of nodule
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is a limitation of Cotton Swab?

A

Low yield if not exudate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

(T/F) A fine needle aspirate CAN replace histopathology

A

False, does not replace histopathology

17
Q

Culture:
When do you add anaerobic and atypical mycobacterial culture plus fungal future?

A

If it is a DEEP infection

18
Q

Culture:
Bacterial will most likely be ___________

A

Aerobic

19
Q

Culture:
What culture do you use for Dermatophytes?

A

Fungal Dermatophyte Test Medium (DTM)
(use Wood lamp)

20
Q

List the Indications for Bacterial Culture:

A
  • Poor response to antimicrobial therapy
  • All chronic deep infections
  • Rods found on cytology
    - Unresponsive bacterial otitis
  • Rule out infectious organism if suspect sterile dz
21
Q
  • Sterile punch biopsy procedure
  • Use the sterile instrument pack
  • Surgically prep the skin & rinse well with saline before biopsy
    What type of culture is this?
A

Deep Skin Culture

22
Q

(T/F) Do not biopsy ulcerated areas for culture

A

True

23
Q
  1. Sterilize the collection site from saprophytic fungi by gently wiping with 70% alcohol and let dry
  2. Remove hair and/or scale from the margin of the lesion or nails with sterile hemostats and place either in a sterile collection vial or inoculate into Sabouraud’s dextrose agar
  3. A yellow-to-red color change in media with concurrent colony growth, typically occurs within 7-10 days
  4. For fungal identification, collect macroconidia from the colony using adhesive tape, place the tape on a slide with several drops of lactophenol cotton blue, cover slip the sample and examine under 40x
  5. Best to send out to a lab

This method is from:

A

Routine Fungal – DTM

24
Q

Therapeutic Trials:
- Rule out/in the following parasitic diseases
- Flea allergy dermatitis
- Demodex gatoi
- Sarcoptiform mites (scabies, Notoedres, Cheyletiella)

A

Insecticidal trial

25
Q

Therapeutic Trials:
- Cutaneous adverse food reaction

A

Food allergy dietary trial

26
Q

While doing a therapeutic trial, part of it needs to be performed without the use of what?

A
  • Antipruritics
    • Glucocorticoids
    • Antihistamines
    • Topical therapy
27
Q

What are the 2 reasons for preforming a skin biopsy?

A
  1. For histopathology
  2. For tissue culture
28
Q

When do you biopsy for Histopathology?

A
  1. Suspect neoplasia
  2. Ulcerative disease (not ulcerative lesions)**
  3. Mucosal lesions
  4. Footpad lesions
  5. Nodular lesions
  6. Unknown disease after work-up
  7. Rule out disease
29
Q

In biopsy techniques for histopathology, you _______ (do/do not) prep, scrub, or disturb the lesions

A

do not

30
Q

(T/F) Grasping the epidermis DAMAGES the epidermal structures

A

True

31
Q

List the three ways to take a Histopathology:

A
  1. Punch biopsy (most common)
  2. Wdge biopsy
  3. Amputation of third phalanx
32
Q

What do you biopsy for histopathology?

A
  • Acute (new) lesions
  • Primary lesions
    * Pustules – very transient
    * Papules
    * Vesicles – very transient
    * Petechiae
    * Erythematous macules
  • Entire lesion
  • Multiple lesions
  • Center of the area of alopecia
33
Q

What do you NOT biopsy for histopathology?

A
  • If secondary infection
  • Severely traumatized skin
  • Chronic lesions
  • Large areas of normal skin
34
Q

(T/F) Biopsy doesn’t have to include all layers of the skin

A

False, it does have to include all layers

35
Q

The development of molecular techniques has resulted in tests that identify:
1. Inherited mutations that lead to inappropriate drug metabolism
2. Mutations that lead to genetic diseases of the hair color, adnexa, skin, and muscle

A

Tests of Non-Cutaneous Organs

36
Q

It is used to collect surface cytology

A

10 blade

Integumentary Sys (6 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions