Module 1: Bone Biology, Skeletal Development & Anatomical Terms Flashcards
What is the structure of bone?
90% Protein (Collagen) and Mineral (hydroxyapatite)
How are bones formed?
Osteoblasts
Where are osteoblasts found?
Underneath the periosteum of bone
Periosteum
Thin membrane on the outer surface of bone
Osteoclasts
Resorb or remove bone that is unnecessary
Wolff’s Law
Bone is laid down where it is needed and resorbed where it is not
What is the first step in a forensic investigation?
Determining remains are bone vs other material
What is the second step in a forensic investigation?
Determining animal vs human bone
How do you differentiate bone from non-bone?
Gross inspection, screening with 1/4” mesh, flotation, etc.
Taphonomy
Environmental or human changes after death
Radiography
Bone is usually more radio-opaque than sticks, plastic, other materials, so x-raying remains is a good way to sift through remains
General microscopic analysis
Demonstrates texture and structure of bone that can be useful in ID
ALS (Alternative light source)
Using shortwave light to ID bone-bone fluoresces
Histological analysis
Can show microstructures which can help identify bone and tooth. Requires thin sectioning of bone.
Elemental analysis
SEM/EDS or XRF to assess calcium and phosphorus content of materials
Human bone
Textured, concentrated trabecular structures at ends of bones
Mammal bone
Smooth in appearance, slight wood grain pattern, more homogeneous trabecular distribution
Bird bone
smooth appearance, thin/less dense trabeculae
Reptile bone
Smooth appearance, little to no trabecular bone, dense cortical bone
When remains are fragmentary, what is the most helpful way to analyze materials?
Microscopic analysis
Non-human bone
Usually non-Haversian and more linear. Fibrolamellar, laminar, or plexiform bone.
How do mammal bones grow
In diameter very rapidly, as opposed to in length slowly
Plexiform bone
Brick-like appearance or linear
Haversian systems
Near muscle attachment sites in large mammals, consist of concentric rings oriented along the long axis of the bone
Fibrolamellar bone
Layered/sandwiched and consisting of alternating Haversian and lamellar banding patterns, rarely in humans
PRIA
radioimmunoassay, used to ID species proteins/DNA
Osteocytes
Living bone cells. Growing bones are high in these, resulting in woven appearance “woven bone”
Immature bone
“Woven bone.” Random collagen fibers, porous and disorganized
Mature bone
Organized, more uniform
Where is immature bone found after growth?
Fracture repairs and bone tumors
Compact bone (dense/cortical bone)
Found in the outer shaft of long bones or joints. Contains Haversian canals for nutrients to flow.
Subchondral bone
Found on the joint surfaces, covered by cartilage. Smooth and shiny appearance
Trabecular bone (spongy/cancellous)
Fed by blood vessels within marrow cavity. Porous/honeycomb structure. Tightly packed at joint ends of bone. Strut system for support while making bones not heavy
Bone growth
Deposition of bone on preexisting surface or tissue
Intramembranous ossification
Ossification that occurs within a tissue membrane. Predominantly in skull bones
Endochondral ossification
Occurs along a cartilaginous model. Base of skull and post-cranial elements
Long bones
Grow in length and width
Appositional growth
Allows the shaft to enlarge
Epiphyseal plates
Allow for longitudinal growth
Diaphysis
Bone shaft
Epiphysis
End/joint surfaces
Physis
Growth plate
Where does bone growth occur?
diaphyseal side of plate until full shaft length reached, then fusion begins
Cranial
Towards head
Caudal
Towards tail
Rostral
Towards nose when referencing skull
Ventral
Towards belly or ground, down
Dorsal
Towards back or sky, up
Medial
Towards midline
Lateral
Away from midline
Proximal
Near axial skeleton (limbs)
Distal
Farther from axial skeleton (limbs)
Volar
Superior/anterior surface of paw or foot
Plantar
Interior/posterior surface of paw or foot
Axial
Head, ribs, vertebra
Appendicular
Appendages, arms, legs, sockets
Mesial
Towards midline or central incisors
Distal
Away from the midline
Lingual
Towards tongue
Labial
Towards lips (anterior teeth/incisors)
Buccal
Towards cheeks (posterior teeth/molars)
Interproximal
Between two teeth
Occlusal
Chewing surface, incisal surface on incisors