Mod 6 Ch. 21 Flashcards
Which of the following is a use of a microorganism in biotechnology?
All of the above
Production of medicines
Food fermentation
Biological control
What is the key reason why the A and B chains of insulin are made as fusion proteins with beta-galactosidase?
To prevent their degradation
Which of the following was the first living organism to be patented?
An oil-eating bacterium
When a cloned gene integrates into a genome by nonhomologous recombination, the end result is..
Gene addition
What is a chimera?
An organism that contains cells from two different individuals
One strategy for producing a protein in the milk of a cow is to place the coding sequence of the gene of interest next to a ___ and then inject the gene into a ___.
Beta-lactoglobulin promoter, cow oocyte
During mammalian reproductive cloning, ___ is fused with ___.
A somatic cell, an enucleated egg cell
Which of the following is a key feature of stem cells?
- have the ability to divide
- have the ability to differentiate
When A. tumefaciens is used to make a transgenic plant, a gene of interest is inserted into a ___, which is first transformed into ___. The ___ is then transferred to a plant.
T-DNA vector, A. tumefaciens, T DNA carrying the gene of interest
In addition to T-DNA vectors, other technologies to produce transgenic plants include..
- biolistic gene transfer
- micro injection
- electroporation
A method of introducing a cloned gene into cells for gene therapy is via..
- liposomes
- retroviral vectors
Which of the following best describes the approach that was used in the first gene therapy trial for SCID?
Lymphocytes were removed from a SCID patient, the normal ADA gene was transferred into the lymphocytes via a retrovirus, and then the lymphocytes were returned to the patients body
