Mod 2 Lec 5 - growth kinetics Flashcards
what key things do bact need to grow
carbon
nitrogen (needed in prtoein synth)
S, P, Mg etc (and trace elements)
Growth factors (usually for pathogenic bacteria can use blood agar plate, e.g. vitamins, nucleotides etc)
name of ring that triggers division
FtsZ
what type of bacteria is MreB present in
rod shaped bacteria
role of autolysin
breaks the b1-4 linkage between NAM and NAG
name the enzyme that adds new peptidoglycan units after bacterial separation
Transglycosylase
what type of bacfteria is FtsZ in
cocci and rod shaped
name of protein that recruits the enzymes involved in destroying and resynthesising of peptidoglycan layer
MreB
wht’s the culture called when no fresh medium is added to it
batch culture
‘closed’ culture
4 phases of bact growth
lag
log
stationary
death
why is there no initial increase when cells put into fresh medium
cells making new components
getting ready to grow
explains the lag phase= cells old, readjusting to new medium
2 ways of measuring bacterial growith
viable count
optical density - spectrophotometry (shows rate only)
what is cardinal temperatures
minimum (Tmin ), optimum (Topt ), maximum (T max )
4 temperature clsases
psychrophiles (under 15)
mesophiles (15-45)
thermophiles (45 - 80)
hypothermophiles (80-100)
2 main ways psychrophiles adapted to low temps
unsaturate dfatty acid membrane
more alpha helices in proteins = more flexibility
2 main ways thermophiles adapted to low temps
saturated fatty acid membrane
AA changes, heat resistant folding in proteins
