Misc GN Orgs- Needy GNB

Question 1

What is Brucella “needy”?

Answer 1

It has an extended incubation time of three weeks

Question 2

What is Campylobacter “needy”?

Answer 2

Microaerophilic and 42 degrees

Question 3

What is Helicobacter “needy”?

Answer 3

Doesn’t grow well
Microaerophilic
Usually urine test

Question 4

What is Vibrio “needy”?

Answer 4

Most are halophilic

Question 5

What is Legionella “needy”?

Answer 5

Requires L-cysteine and iron for growth

can not survive in dry environments

Question 6

Bordetella

Answer 6

Requires special media:

Bordet-Gengou or Regan-Lowe for 7-10 days

Question 7

What is Francisella “needy”?

Answer 7

Requires special agar:

Blood cysteine glucose agar with thiamine

Question 8

List the Needy GNB

Answer 8

Brucella
Campylobacter
Helicobacter
Vibrio
Legionella
Bordetella
Francisella

Question 9

What is Brucella’s air status?

Answer 9

Aerobic

Question 10

Brucella gram stain?

Answer 10

GNCB

Question 11

What disease doese Brucella cause

Answer 11

zoonosis
Brucellosis or Undulant fever
–recuring fever that rises and falls

Question 12

Brucella is acquired by

Answer 12

Ingestion of contaminated dairy
Inhalation of aerosolized particles
Abrasion from handling an infected animal
Can be a lab acquired infection

Question 13

Where is Brucella most commonly isolated from?

Answer 13

human blood

Question 14

What level safety hood is required for Brucella?

Answer 14

Level 3

Question 15

Campylobacter jejuni gram stain?

Answer 15

Motile, curved GNB

very faint staining and seagull wings

Question 16

Campylobacter jejuni air status?

Answer 16

Microaerophilic–85% N, 5% O, 10% CO2

Question 17

What is the MCC of bacterial diarrheal disease in the US?

Answer 17

Campylobacter jejuni

Question 18

What temp does Campylobacter jejuni grow at?

Answer 18

42 C

Question 19

Campylobacter jejuni is oxidase

Answer 19

positive

Question 20

Helicobacter pylori is urease

Answer 20

strongly positive

Question 21

What disease does Helicobacter pylori cause?

Answer 21

peptic ulcers and it is the only organism that can survive in the stomach

Question 22

What is the only organism that can survive in the stomach?

Answer 22

Helicobacter pylori

Question 23

How is Helicobacter pylori infection MC ided?

Answer 23

via gastric biopsy or urea test; Clo Test

Question 24

Gram stain of Helicobacter pylori?

Answer 24

Rarely done; similar to Campy, but can have U shape

Question 25

What are Helicobacter pylori growth requirements??

Answer 25

similar to CAmpylobacter

Question 26

Vibrio cholera disease

Answer 26

rice water stools due to potent enterotoxin

Question 27

Vibrio cholera gram stain

Answer 27

"comma" shaped

Question 28

Vibrio cholera environment?

Answer 28

Natural inhabitant of salt water

Question 29

Vibrio cholera is halophilic

Answer 29

It needs salt to grow and this is why it is needy

Question 30

Vibrio cholera acquired by

Answer 30

Contaminated water or seafood

Question 31

Strains of Vibrio other than Vibrio cholera cause what disease MC?

Answer 31

wound infections

Question 32

What special agar is recommended for isolation?

Answer 32

TCBS agar

Question 33

Does Vibrio cholera grow on BAP?

Answer 33

Yes and is sometimes beta hemolytic

Question 34

What is the appearance of Vibrio cholera on TCBS agar?

Answer 34

Yellow colonies b/c it is a sucrose fermenter

Question 35

Legionella pneumophilia causes?

Answer 35

Legionairre's disease

Question 36

Legionella pneumophilia is MC acquired by?

Answer 36

via inhalation from water systems such as air conditioners or plumbing systems

Question 37

How is Legionella pneumophilia transmitted?

Answer 37

only from contaminated water inhalation | No person to person transmission

Question 38

Media of choice for Legionella pneumophilia?

Answer 38

Buffered charcol yeast extract (BCYE)

Question 39

Does Legionella pneumophilia grow on regular media?

Answer 39

No. | Must have L-cysteine

Question 40

What are the risk for acquiring Legionella pneumophilia infection?

Answer 40

Older male smoker

Question 41

What tests other than plate growth is used for iding Legionella pneumophilia?

Answer 41

Urine antigen test | DFA

Question 42

What test is best for detecting Bordetella pertussis?

Answer 42

PCR

Question 43

What special media is required for Bordetella pertussis?

Answer 43

Bordet-Gengou or Regan-Lowe

Question 44

Gram stain Bordetella pertussis?

Answer 44

faint, tiny GNCB

Question 45

What is the optimal specimen for Bordetella pertussis?

Answer 45

Nasopharyngeal

Question 46

How long is incubation for Bordetella pertussis?

Answer 46

7 to 20 day incubation

Question 47

Virulence factors Bordetella pertussis?

Answer 47

capsule endotoxin pertussis toxin

Question 48

What disease is caused by Bordetella pertussis?

Answer 48

whooping cough

Question 49

Bordetella pertussis has an strong affinity for what cells?

Answer 49

ciliated epithelial cells in the mucous membranes of the respiratory tract

Question 50

How contagious is Bordetella pertussis?

Answer 50

VERY

Question 51

How can Bordetella pertussis infection be prevented?

Answer 51

Vaccine! boosters at 5 and 11

Question 52

Gram stain for Francisella tularemia?

Answer 52

Pleomorphic GNB | Very faint staining

Question 53

What disease does Francisella tularemia cause?

Answer 53

Tularemia

Question 54

What are the S/S of Tularemia?

Answer 54

Ulcer forms are the site of infection and stays for months | fever, chills, lymphoadenopathy

Question 55

How is Francisella tularemia acquired?

Answer 55

transmitted by biting flies and ticks

Question 56

Why is Francisella tularemia dangerous?

Answer 56

CAN CAUSE LAB ACQUIRED INFECTION and | BIOTERRISM

Question 57

What virulence factors does Francisella tularemia have?

Answer 57

Capsule

Question 58

Francisella tularemia oxidase reaction?

Answer 58

Negative

Question 59

Francisella tularemia catalase reaction?

Answer 59

Catalase Positive

Question 60

What is the special agar for Francisella tularemia?

Answer 60

Blood cysteine glucose agar with thiamine

Question 61

Will Francisella tularemia grow on BAP or Choc?

Answer 61

On both, but will be very tiny and faint staining