Misc GN Orgs- Needy GNB Flashcards by Rebecca Hunter

What is Brucella “needy”?

It has an extended incubation time of three weeks

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What is Campylobacter “needy”?

Microaerophilic and 42 degrees

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What is Helicobacter “needy”?

Doesn’t grow well
Microaerophilic
Usually urine test

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What is Vibrio “needy”?

Most are halophilic

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What is Legionella “needy”?

Requires L-cysteine and iron for growth

can not survive in dry environments

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Bordetella

Requires special media:

Bordet-Gengou or Regan-Lowe for 7-10 days

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What is Francisella “needy”?

Requires special agar:

Blood cysteine glucose agar with thiamine

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List the Needy GNB

Brucella
Campylobacter
Helicobacter
Vibrio
Legionella
Bordetella
Francisella

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What is Brucella’s air status?

Aerobic

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Brucella gram stain?

GNCB

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What disease doese Brucella cause

zoonosis
Brucellosis or Undulant fever
–recuring fever that rises and falls

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Brucella is acquired by

Ingestion of contaminated dairy
Inhalation of aerosolized particles
Abrasion from handling an infected animal
Can be a lab acquired infection

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Where is Brucella most commonly isolated from?

human blood

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What level safety hood is required for Brucella?

Level 3

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Campylobacter jejuni gram stain?

Motile, curved GNB

very faint staining and seagull wings

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Campylobacter jejuni air status?

Microaerophilic–85% N, 5% O, 10% CO2

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What is the MCC of bacterial diarrheal disease in the US?

Campylobacter jejuni

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What temp does Campylobacter jejuni grow at?

42 C

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Campylobacter jejuni is oxidase

positive

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Helicobacter pylori is urease

strongly positive

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What disease does Helicobacter pylori cause?

peptic ulcers and it is the only organism that can survive in the stomach

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What is the only organism that can survive in the stomach?

Helicobacter pylori

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How is Helicobacter pylori infection MC ided?

via gastric biopsy or urea test; Clo Test

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Gram stain of Helicobacter pylori?

Rarely done; similar to Campy, but can have U shape

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What are Helicobacter pylori growth requirements??

similar to CAmpylobacter

Vibrio cholera disease

rice water stools due to potent enterotoxin

Vibrio cholera gram stain

"comma" shaped

Vibrio cholera environment?

Natural inhabitant of salt water

Vibrio cholera is halophilic

It needs salt to grow and this is why it is needy

Vibrio cholera acquired by

Contaminated water or seafood

Strains of Vibrio other than Vibrio cholera cause what disease MC?

wound infections

What special agar is recommended for isolation?

TCBS agar

Does Vibrio cholera grow on BAP?

Yes and is sometimes beta hemolytic

What is the appearance of Vibrio cholera on TCBS agar?

Yellow colonies b/c it is a sucrose fermenter

Legionella pneumophilia causes?

Legionairre's disease

Legionella pneumophilia is MC acquired by?

via inhalation from water systems such as air conditioners or plumbing systems

How is Legionella pneumophilia transmitted?

only from contaminated water inhalation | No person to person transmission

Media of choice for Legionella pneumophilia?

Buffered charcol yeast extract (BCYE)

Does Legionella pneumophilia grow on regular media?

No. | Must have L-cysteine

What are the risk for acquiring Legionella pneumophilia infection?

Older male smoker

What tests other than plate growth is used for iding Legionella pneumophilia?

Urine antigen test | DFA

What test is best for detecting Bordetella pertussis?

PCR

What special media is required for Bordetella pertussis?

Bordet-Gengou or Regan-Lowe

Gram stain Bordetella pertussis?

faint, tiny GNCB

What is the optimal specimen for Bordetella pertussis?

Nasopharyngeal

How long is incubation for Bordetella pertussis?

7 to 20 day incubation

Virulence factors Bordetella pertussis?

capsule endotoxin pertussis toxin

What disease is caused by Bordetella pertussis?

whooping cough

Bordetella pertussis has an strong affinity for what cells?

ciliated epithelial cells in the mucous membranes of the respiratory tract

How contagious is Bordetella pertussis?

VERY

How can Bordetella pertussis infection be prevented?

Vaccine! boosters at 5 and 11

Gram stain for Francisella tularemia?

Pleomorphic GNB | Very faint staining

What disease does Francisella tularemia cause?

Tularemia

What are the S/S of Tularemia?

Ulcer forms are the site of infection and stays for months | fever, chills, lymphoadenopathy

How is Francisella tularemia acquired?

transmitted by biting flies and ticks

Why is Francisella tularemia dangerous?

CAN CAUSE LAB ACQUIRED INFECTION and | BIOTERRISM

What virulence factors does Francisella tularemia have?

Capsule

Francisella tularemia oxidase reaction?

Negative

Francisella tularemia catalase reaction?

Catalase Positive

What is the special agar for Francisella tularemia?

Blood cysteine glucose agar with thiamine

Will Francisella tularemia grow on BAP or Choc?

On both, but will be very tiny and faint staining