Misc GN Orgs- Needy GNB Flashcards
What is Brucella “needy”?
It has an extended incubation time of three weeks
What is Campylobacter “needy”?
Microaerophilic and 42 degrees
What is Helicobacter “needy”?
Doesn’t grow well
Microaerophilic
Usually urine test
What is Vibrio “needy”?
Most are halophilic
What is Legionella “needy”?
Requires L-cysteine and iron for growth
can not survive in dry environments
Bordetella
Requires special media:
Bordet-Gengou or Regan-Lowe for 7-10 days
What is Francisella “needy”?
Requires special agar:
Blood cysteine glucose agar with thiamine
List the Needy GNB
Brucella Campylobacter Helicobacter Vibrio Legionella Bordetella Francisella
What is Brucella’s air status?
Aerobic
Brucella gram stain?
GNCB
What disease doese Brucella cause
zoonosis
Brucellosis or Undulant fever
–recuring fever that rises and falls
Brucella is acquired by
Ingestion of contaminated dairy
Inhalation of aerosolized particles
Abrasion from handling an infected animal
Can be a lab acquired infection
Where is Brucella most commonly isolated from?
human blood
What level safety hood is required for Brucella?
Level 3
Campylobacter jejuni gram stain?
Motile, curved GNB
very faint staining and seagull wings
Campylobacter jejuni air status?
Microaerophilic–85% N, 5% O, 10% CO2
What is the MCC of bacterial diarrheal disease in the US?
Campylobacter jejuni
What temp does Campylobacter jejuni grow at?
42 C
Campylobacter jejuni is oxidase
positive
Helicobacter pylori is urease
strongly positive
What disease does Helicobacter pylori cause?
peptic ulcers and it is the only organism that can survive in the stomach
What is the only organism that can survive in the stomach?
Helicobacter pylori
How is Helicobacter pylori infection MC ided?
via gastric biopsy or urea test; Clo Test
Gram stain of Helicobacter pylori?
Rarely done; similar to Campy, but can have U shape
What are Helicobacter pylori growth requirements??
similar to CAmpylobacter
Vibrio cholera disease
rice water stools due to potent enterotoxin
Vibrio cholera gram stain
“comma” shaped
Vibrio cholera environment?
Natural inhabitant of salt water
Vibrio cholera is halophilic
It needs salt to grow and this is why it is needy
Vibrio cholera acquired by
Contaminated water or seafood
Strains of Vibrio other than Vibrio cholera cause what disease MC?
wound infections
What special agar is recommended for isolation?
TCBS agar
Does Vibrio cholera grow on BAP?
Yes and is sometimes beta hemolytic
What is the appearance of Vibrio cholera on TCBS agar?
Yellow colonies b/c it is a sucrose fermenter
Legionella pneumophilia causes?
Legionairre’s disease
Legionella pneumophilia is MC acquired by?
via inhalation from water systems such as air conditioners or plumbing systems
How is Legionella pneumophilia transmitted?
only from contaminated water inhalation
No person to person transmission
Media of choice for Legionella pneumophilia?
Buffered charcol yeast extract (BCYE)
Does Legionella pneumophilia grow on regular media?
No.
Must have L-cysteine
What are the risk for acquiring Legionella pneumophilia infection?
Older male smoker
What tests other than plate growth is used for iding Legionella pneumophilia?
Urine antigen test
DFA
What test is best for detecting Bordetella pertussis?
PCR
What special media is required for Bordetella pertussis?
Bordet-Gengou or Regan-Lowe
Gram stain Bordetella pertussis?
faint, tiny GNCB
What is the optimal specimen for Bordetella pertussis?
Nasopharyngeal
How long is incubation for Bordetella pertussis?
7 to 20 day incubation
Virulence factors Bordetella pertussis?
capsule
endotoxin
pertussis toxin
What disease is caused by Bordetella pertussis?
whooping cough
Bordetella pertussis has an strong affinity for what cells?
ciliated epithelial cells in the mucous membranes of the respiratory tract
How contagious is Bordetella pertussis?
VERY
How can Bordetella pertussis infection be prevented?
Vaccine! boosters at 5 and 11
Gram stain for Francisella tularemia?
Pleomorphic GNB
Very faint staining
What disease does Francisella tularemia cause?
Tularemia
What are the S/S of Tularemia?
Ulcer forms are the site of infection and stays for months
fever, chills, lymphoadenopathy
How is Francisella tularemia acquired?
transmitted by biting flies and ticks
Why is Francisella tularemia dangerous?
CAN CAUSE LAB ACQUIRED INFECTION and
BIOTERRISM
What virulence factors does Francisella tularemia have?
Capsule
Francisella tularemia oxidase reaction?
Negative
Francisella tularemia catalase reaction?
Catalase Positive
What is the special agar for Francisella tularemia?
Blood cysteine glucose agar with thiamine
Will Francisella tularemia grow on BAP or Choc?
On both, but will be very tiny and faint staining