Anaerobes Flashcards

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1
Q

Anaerobe definition?

A

organisms that cannot grow in the presence of oxygen. Some species can grow in minimal oxygen (2-5%) and others are inhibited by as little as 0.5%.

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2
Q

How many bacteria are in the Anaerobes group?

A

5 Gram Neg Bacilli
7 Gram Pos Bacilli
3 Gram Pos Cocci
1 Gram Neg Cocci

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3
Q

Which anaerobes are aerotolerant?

A

Actinomyces and Lactobacillus are aerotolerant GPB because they will grow in both environments

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4
Q

What are the anaerobic 5 GNB?

A
Bacteroides fragilis
Fusobacterium nucleatum
Fusobacterium necrophorum
Prevotella intermedia
Porphyromonas asaccharolyticus
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5
Q

What are the 7 Anaerobic GPB?

A
Clostridium tetani
Clostridium botulini
Clostridium perfringens
Clostridium difficile
Propionbacterium
Actinomyces
Lactobacillus
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6
Q

What are the 3 Anaerobic GPC?

A

Peptostreptococcus
Peptinophilus asaccharolyticus
Finegoldia magna

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7
Q

What is the 1 anaerobic GNC?

A

Veillonella

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8
Q

What is the MCC of anaerobic infection?

A

Bacteroides fragilis

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9
Q

Bacteroides fragilis causes what type of infection?

A

Extremly virulent
Causes tissue necrosis
MCC of infection is after abdominal trauma
but it can also cause skin infections

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10
Q

Bacteroides fragilis gram stain?

A

GNB with rounded ends

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11
Q

Bacteroides fragilis important biochem tests?

A

grows in 20% bile and bile esculin positive

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12
Q

Bacteroides fragilis grows as a shiny gray colony on what media?

A

ABAP

Also Grows on BBE and LKV

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13
Q

MC Clostridium isolated in lab?

A

Clostridium perfringens

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14
Q

Clostridium perfringens causes what type of infections?

A

myonecrosis (gas gangrene)
food poisoning that is not usu severe, self-limiting
infections where there is abdominal trauma

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15
Q

Contributes to Clostridium perfringens virulence?

A

produces several toxins and lots of gas

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16
Q

Clostridium perfringens gram stain?

A

LG GPB that line up in boxcar formation

17
Q

Clostridium perfringens on BAP?

A

double zone of hemolysis and anaerobic BAP

18
Q

Clostridium perfringens on Egg yolk agar?

A

demonstrates a yellow halo because it is lecithinase + and lipase -

19
Q

Fusobacterium nucleatum Gram stain?

A

Thin GNB with tapered ends

20
Q

Fusobacterium necrophorum Gram stain?

A

pleomorphic spindles GNB with pointed ends

21
Q

Prevotella intermedia Gram stain?

A

GNB that is pigmented and saccharolytic

22
Q

Porphyromonas asaccharolyticus Gram stain?

A

GNB that is also pigmented, but assaccharolytic

23
Q

Clostridium tetani Gram stain?

A

GPB with “tennis racket” morphology

24
Q

Clostridium botulini Gram stain?

A

LG GPB–chiclets

25
Q

Clostridium difficile Gram stain?

A

GPB

26
Q

Propionibacterium Gram stain?

A

non spore forming GP bacillus

“pom-poms”

27
Q

Peptostreptococcus Gram stain?

A

Anaerobic Gram Positive Cocci

28
Q

Parvimonas micra Gram stain?

A

GP cocci

29
Q

Veionella Gram stain?

A

gram negative cocci

can be seen in pairs, chains