Anaerobes

Question 1

Anaerobe definition?

Answer 1

organisms that cannot grow in the presence of oxygen. Some species can grow in minimal oxygen (2-5%) and others are inhibited by as little as 0.5%.

Question 2

How many bacteria are in the Anaerobes group?

Answer 2

5 Gram Neg Bacilli
7 Gram Pos Bacilli
3 Gram Pos Cocci
1 Gram Neg Cocci

Question 3

Which anaerobes are aerotolerant?

Answer 3

Actinomyces and Lactobacillus are aerotolerant GPB because they will grow in both environments

Question 4

What are the anaerobic 5 GNB?

Answer 4

Bacteroides fragilis
Fusobacterium nucleatum
Fusobacterium necrophorum
Prevotella intermedia
Porphyromonas asaccharolyticus

Question 5

What are the 7 Anaerobic GPB?

Answer 5

Clostridium tetani
Clostridium botulini
Clostridium perfringens
Clostridium difficile
Propionbacterium
Actinomyces
Lactobacillus

Question 6

What are the 3 Anaerobic GPC?

Answer 6

Peptostreptococcus
Peptinophilus asaccharolyticus
Finegoldia magna

Question 7

What is the 1 anaerobic GNC?

Answer 7

Veillonella

Question 8

What is the MCC of anaerobic infection?

Answer 8

Bacteroides fragilis

Question 9

Bacteroides fragilis causes what type of infection?

Answer 9

Extremly virulent
Causes tissue necrosis
MCC of infection is after abdominal trauma
but it can also cause skin infections

Question 10

Bacteroides fragilis gram stain?

Answer 10

GNB with rounded ends

Question 11

Bacteroides fragilis important biochem tests?

Answer 11

grows in 20% bile and bile esculin positive

Question 12

Bacteroides fragilis grows as a shiny gray colony on what media?

Answer 12

ABAP

Also Grows on BBE and LKV

Question 13

MC Clostridium isolated in lab?

Answer 13

Clostridium perfringens

Question 14

Clostridium perfringens causes what type of infections?

Answer 14

myonecrosis (gas gangrene)
food poisoning that is not usu severe, self-limiting
infections where there is abdominal trauma

Question 15

Contributes to Clostridium perfringens virulence?

Answer 15

produces several toxins and lots of gas

Question 16

Clostridium perfringens gram stain?

Answer 16

LG GPB that line up in boxcar formation

Question 17

Clostridium perfringens on BAP?

Answer 17

double zone of hemolysis and anaerobic BAP

Question 18

Clostridium perfringens on Egg yolk agar?

Answer 18

demonstrates a yellow halo because it is lecithinase + and lipase -

Question 19

Fusobacterium nucleatum Gram stain?

Answer 19

Thin GNB with tapered ends

Question 20

Fusobacterium necrophorum Gram stain?

Answer 20

pleomorphic spindles GNB with pointed ends

Question 21

Prevotella intermedia Gram stain?

Answer 21

GNB that is pigmented and saccharolytic

Question 22

Porphyromonas asaccharolyticus Gram stain?

Answer 22

GNB that is also pigmented, but assaccharolytic

Question 23

Clostridium tetani Gram stain?

Answer 23

GPB with “tennis racket” morphology

Question 24

Clostridium botulini Gram stain?

Answer 24

LG GPB–chiclets

Question 25

Clostridium difficile Gram stain?

Answer 25

GPB

Question 26

Propionibacterium Gram stain?

Answer 26

non spore forming GP bacillus

“pom-poms”

Question 27

Peptostreptococcus Gram stain?

Answer 27

Anaerobic Gram Positive Cocci

Question 28

Parvimonas micra Gram stain?

Answer 28

GP cocci

Question 29

Veionella Gram stain?

Answer 29

gram negative cocci

can be seen in pairs, chains