MIDTERM LAB: BIOCHEMICAL TESTS

Question 1

ferments glucose and maltose

Answer 1

N. meningitidis

Question 2

ferments glucose

Answer 2

N. gonorrhoeae

Question 3

Gram-negative bacteria
- Has:

Answer 3

COFFE
o Capsular antigen
o O antigen
o Flagella
o Fermenters
o Enterobacteriaceae

Question 3

• mnemonics for Enterobacteriaceae
• usually have capsule

Answer 3

MESSY SPECK

Question 4

Enterobacteriaceae:

Answer 4

MESSY SPECK
o Morganella
o Escherichia coli
o Shigella
o Salmonella
o Yersinia
o Serratia
o Proteus
o Enterobacter
o Citrobacter
o Klebsiella

Question 5

CLINICAL SIGNS:

Answer 5

1. MICROSCOPY
2. CULTURE
3. BIOCHEMICAL TEST

SPECIAL TEST
4. SEROLOGICAL TEST
5. MOLECULAR TECHNIQUES

Question 6

BIOCHEMICAL TESTS:

Answer 6

1. Triple Sugar Iron (TSI)/ Kligler’s Iron Agar (KIA)
2. Decarboxylase Test
3. Lysine Iron Agar (LIA)
4. IMViC Reaction
5. Urease Test
6. Motility test
7. Carbohydrate Fermentation
8. CHO Fermentation Using Broth Fermentation Media
9. ONPG (O-nitrophenyl-beta-D-galactopyranoside)
10. Nitrate Reduction Test
11. Phenylalanine Deaminase Test
12. Gelatin Hydrolysis