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Clinical Chemistry > Midterm > Flashcards

Midterm Flashcards

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0
Q

Conductance

A

Siemens ( S )

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1
Q

Activity( radionuclide )

A

Becquerel ( Bq )

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2
Q

Resistance

A

Ohms

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3
Q

Substance conc.

A

Mols per cubic meter

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4
Q

Volume

A

Cubic meter

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5
Q

Are obtain by combining 2 or more units by multiplication or division

A

SI derived units

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6
Q

States that the analyze is directly proportional to the amount of light it absorbs

A

Beer’s law

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7
Q

Is the term used to describe the monochromatic light this is absorbed by the sample and transmittance describes the light that passes through the sample

A

Absorbable ( OD )

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8
Q

Visual colorimetry which solely on the human eye

A

Dubosq colorimeter

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9
Q

A device for selecting a narrow band of wavelengths from a continuous spectrum

A

Monochromator

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10
Q

When the absorbance increases exponentially with an increase in the light path this law is followed

A

Lambert law

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11
Q

Incorporation of these two laws together with the contribution of blank

A

Bouguer

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12
Q

Formula of absorbance OD

A

A= 2 - log%T

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13
Q

Continuous spectrum examples

A

Filter ,Prism and Diffraction grating

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14
Q

Photoelectric colorimetry which employs a photoelectric device

A

Leitz photrometer

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15
Q

LambertlAw and contribution of bouguer

A

A= abc A= is for absorbance
a= absorptivity of the substance being measured
b= light in cm
c= conc. of the measured substance

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16
Q

Beer-Lambert law formula

A

Au x Cs /As = Cu

17
Q

Two types of beer law

A

Duboscq colorimeter and Lietz photrometer

18
Q

This formula applied to assay exhibit linear relationship between changes in absorbance with changes in conc to calculate the conc of unknown

A

Au x Cs /As = Cu

19
Q

It minimizes stray light emitted by lamp and prevent scattered light from entering the monochromator

A

Entrance slit

20
Q

Does not provide a continuous spectrum ,emitting radiation at a specific wavelength

A

Mercury vapor lamp

21
Q

It controls the amount of emergent light that passes through the event

A

Exit slit

22
Q

This provide the radiant energy

A

Light source

23
Q

NIR

A

Near infrared

24
Q

Are used for work in the UV region with 200-375 nm range

A

Hydrogen and Deuterium lamps

25
Q

It sharply isolates specific wavelength of light which will made to pass the curvets

A

Monochromator

26
Q

Responds to the radiant energy it absorbs by emitting electrons in a proportional amount to the initial light absorbed

A

Photomultiplier tube

27
Q

Refers to the ability of the test to detect the smallest amount of the analyte In a solution or sample

A

Sensitivity

28
Q

Is a soln of a particular analyte of known characteristics and known value

A

Standard

29
Q

It holds the cuvet holding a soln containing the analytes to be measured

A

Cuvet holder

30
Q

Is the measurement of the amount of light scattered by particles in suspension

A

Nephelometry

31
Q

It converts light signal into an electral signal

A

Detector

32
Q

Are design with 256-2048 photodiode that are arrange in linear fashion

A

Photodiode array detector

33
Q

Cuvet holder for strongly acidic solution

A

Aluminosilicate glass cuvet

34
Q

It provides the actual value or numerical data needed in the calculation of the value of analyte present in the sample

A

Read out device

35
Q

Detector can be detected by

A

Galvanometer

36
Q

Is the measurement of the amount of light blocked by particulate matter

A

Turbidimetry

37
Q

A iron plate and a layer of selenium are partial description f the composition

A

Photocell or barrier layer cell

38
Q

Cuvet should be used for assays using wavelength range UV region 200-380

A

Plastic or quartz cuvet

39
Q

Occurs when a molecule absorbs light of a particular short wavelength and is thereby stimulated to emit light of longer wavelength

A

Fluorescence

40
Q

As light from a radiant energy source passes through an interference filter it is projected to the slide at a 45 degree angle

A

Reflectance spectrophotometry

Clinical Chemistry (8 decks)

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