Lipids Flashcards
it is the only phospholipid in membranes that is not derived from an amino alcohol .Name the amino alcohol
Sphingomyelin ,Sphingosine
Other name for Lecithin
Phosphatidyl choline
.Are primary source of fuel , they provide stability to cell membrane and allow transmembrane transport
Lipids
Reference value of Phospholipids
150-380 mg/dl
CF for Phospholipids
0.01 g/L
Simplest form of TAG
Triolein
How many FFA does Phospholipids have?
2 FFA
It is the only lipids that does not serve as source of fuel
Cholesterol
Phospholipids in the Lungs are produced by __________ in the form of ________ .
Pneumocyte type 2 , Lamellar Bodies
2 Forms of Cholesterol
Cholesterol Ester (CE) 70% , Free Cholesterol (FC) 30 percent
3 Forms of Phospholipids
Lecithin/Phosphatidyl choline 70%
Sphingomyelin 30%
Cephalin 10%
It undergoes esterification of LCAT
CE
Is the Activator of LCAT
Apo A1
Reference value of Chelesterol for High Cholesterol
> 240 mg/dl
Excess cholesterol is re-esterified by the ____
microsomal enzyme acyl or ACAT
CDC reference method for Total Cholesterol
Abell , Levy and Brodie Method
Abell Levy and Brodie Method (ALB) uses ___ for extraction and ______ for saponipication plus _____ for colurimetric.
Hexane , Alcoholic KOH , Liebermanburchardt for Coulirenetric
2 chemical methods use in TC
Leibermanburchardt Method
Salkowski Method
End product of Salkowski Method
Cholestadienyl DisuLfonic Acid (RED)
End product of Leibermanburchardt method
Cholestidienyl Monosulfonic Acid (GREEN)
Rgt. use in Leibermanburchardt
G-laceal Acetic Acid
A-cetic anhydride
C-oncentrated sulfuric acid
One Step Method
Colorimetry PSM (PEARSON , STERN MC GAVACK)
Two Step Method
Extracttion + Colorimetric ( BLOOR)
Three Step Method
Saponification + Extraction + Colorimetric (ABELL KENDAL )
Anticoagulant of choice for TAG and TC
EDTA
