Liver Function Test Flashcards
Plasma level of total protein
0.2 to 0.4 g/dl slightly higher than serum due to fibrinogen
the two test that provide the most useful indices for assessing severity of liver disease
(Synthetic Liver Function ) Serum Albumin and Vit. K dependent coagulation factor
how many mg of bilirubin is produce daily in the healthy adult
200-300mg of bilirubin
How many g of albumin daily is produce by the normal liver
12 g
The anatomic unit or functional unit of the liver
Hepatic Lobule
A test for hepatocellular fxn and potency of bile duct
Bromsulfathalein Dye Excretion Test
Ammonia reference value as serum
19-60 ug/dl or 11-35 mmol/L
name 7 enzymes that are secreted in the Liver
ALP , Aminotransferase , 5’ nucleotidase , GGT , OCT , LAP and LD
Enzyme secreted in the liver that is specific for Alcoholic Hepatitis
GGT
Reference value for Total Protein
6.5-8.3 g/dl
Give 1 Clinical significance or disease associated with Gamma Spike
Multiple myeloma
Give 1 Clinical significance or disease associated with alpha 1 globulin flat curve
Juvenile cirrhosis ( AAT deficiency )
Give 1 Clinical significance or disease associated with Beta gamma bridging
Hepatic Cirrhosis
Give 1 Clinical significance or disease associated with alpha 2 globulin band spike
nephrotic syndrome
Is an alternative test to chemical analysis of serum Total Protein
Refractometry
A Method/ Test in Total Protein that is based on the principle that Cupric ion complex the groups involved in the PEPTIDE BOND forming a violet colored chelates which is proportional to the number of peptide bonds present and reflects TOTAL protein level at 545 nm
Biuret Method
Method in Total Protein that has the HIGHEST ANALYTICAL SENSITIVITY
Folin Ciocalteu
Other name of Folin Ciocalteu
Lowry Method
A Method/ Test in Total Protein that is based on the principle OXIDATION OF PHENOLIC CPDS. such as tyrosine , tryptophan and histidine
Folin-Ciocalteu
According to Kjeldahl 1 g of Nitrogen is equivalent to how many grams of Protein?
6.54 g of Protein
A Method/ Test in Total Protein that is based on the principle that the absorbance of protein at 210nm is due to the absorbance of the peptide bond specific wavelength (280 nm)
UV absorption Method
It is the reference method for Total Protein but not routinely use
Kjeldahl Method
Reference value for Albumin
53-65% or 3.5-5.0 g/dl
Reference value for y-Globulin
12-22% or 0.8-1.6 g/dl
Spikes of a1 , a2 and B globulin bands indicates
Inflammation
Globulin is Soluble to ______ and _____.
Weak Salt soln. and Hydrocarbon solvents
Albumin is Soluble to _____ and ______.
Water and Concentrated salt soln
Albumin is Insoluble to _______, _______ and ______
Saturated salt soln
Highly conc. salt soln
Hydrocarbon solvent
Globulin is insoluble to ____, ____ and ______.
Water , Conc and Saturated salt soln.
Is the hereditary absence of albumin ; inability to synthesize albumin
analbuminemia
Most commonly used dye in for Protein
BCG
Most specific dye for Protein
BCP
True or False the presence of penicillin may cause falsely INCREASE RESULT of serum albumin
False it may cause falsely low serum Albumin
It differentiates intrahepatic disorder from extrahepatic obstructive liver disease
Prothrombin time ( Vit. K response Test)
Preferred specimen for Ammonia Determination
Arterial Blood if Venous blood is use tourniquet should be use minimally, and first clenching and relaxing avoided during collection
Formula for Deta Bilirubin
TotalBilirubin - Indirect Bilirubin + Direct Bilirubin