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HistoPath Lec 2ndYR 2ndSem Midterms > Microtomy > Flashcards

Microtomy Flashcards

1
Q

Expected output after embedding

A

Tissue Block

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2
Q

Purpose of Trimming

A

Even out the edges of tissue block

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3
Q

Ideal shape in trimming

A

Truncated Pyramid

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4
Q

At least __mm of wax should surround the tissue

A

2

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5
Q

other term for Microtomy

A

Sectioning

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6
Q

An indication that your sectioning is correct

A

Tissue Ribbons

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7
Q

Complete ribbons are picked up and transferred to a flotation bath using what utensils?

A

camel hair brush
forceps
fingers

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8
Q

Microtome Principle

A

Spring-balanced or pawl is brought into contact with, and turns the ratchet feed wheel connected to a micrometer screw, which is, in turn rotated, moving the tissue back at a predetermined distance towards the knife for cutting sections at a uniform thickness

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9
Q

Essential parts of a microtome

A

Block holder
Knife Carrier and Knife
Pawl
Ratchet Feed Wheel
Adjustment Screws

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10
Q

Part of microtome that holds the tissue block

A

Block Holder

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11
Q

Part of microtome that does the actual cutting or sectioning of the tissues

A

Knife Carrier and Knife

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12
Q

6 Types of Microtome

A

Rocking Microtome
Rotary Microtome
Slide Microtome
Freezing Microtome
Cryostat/Cold Microtome
Ultrathin Microtome

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13
Q

Simplest Microtome

A

Rocking Microtome (Cambridge)

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14
Q

Who invented the Rocking Microtome

A

Paldwell Trefall (1881)

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15
Q

Tissue thickness for Rocking Microtome

A

10-12 um

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16
Q

Most common microtome

A

Rotary Microtome (Minot)

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17
Q

Tissue thickness for Rotary Microtome

A

3-5 um

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18
Q

Who invented the Rotary Microtome

A

Minot (1885-1886)

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19
Q

Who developed the Sliding Microtome

A

Adams (1789)

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20
Q

2 Types of Sliding Microtome

A

Base-Sledge Microtome
Standard Sliding Microtome

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21
Q

Sliding Microtome:

Electrically driven and ideal for resin-embedded decalcified bone

A

Base-Sledge Microtome

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22
Q

Sliding Microtome:

Knife is moving; the most dangerous type of microtome because of the exposed moving blade

A

Standard Sliding Microtome

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23
Q

Recommended microtome for cutting “extremely” hard and rough tissue blocks

A

Sliding Microtome

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24
Q

Who invented the Freezing Microtome

A

Quickett (1848)

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25
Freezing agent used in Freezing Microtome
Carbon Dioxide
26
Microtome used to cut undehydrated tissues in a frozen state
Freezing Microtome
27
Microtome ideal for fresh tissues that did not undergo fixation down infiltration
Freezing Microtome
28
Microtome used for rapid preparation of urgent tissue biopsies for intraoperative diagnosis
Cryostat
29
Cryostat Chamber temp
-5 to -30C (average fo 20C)
30
Part of cryostat capable of freezing fresh tissue within 2-3 mins
Thermostat
31
Tissue size for Cryostat
4 um
32
Tissue size for Ultrathin Microscope
0.5 um
33
Ultrathin Microtome Medium
Plastic
34
Microtome for electron microscopy and tissues fixed with osmic acid
Ultrathin Microtome
35
What knife does Ultrathin Microtome use
Diamond Knife
36
Tissue size of paraffin sections
4-6 um
37
Tissue size of Celloidin sections
10-15 um
38
Celloidin block and sections are kept moist using what
70% Alcohol
39
3 Knives in Microtomes
Plane-Concave Biconcave Plaen-Wedge
40
Plane Concave Knife length
25mm
41
Biconcave Knife length
120mm
42
Plane-Wedge knife length
100mm
43
Microtome knife where one side is flat and the other side is concave
Plance-Concave
44
Microtome Knife where both sides are concave
Biconcave
45
Microtome Knife where both sides are straight
Plane-Wedge
46
Embedding medium for Plane-Concave knives that are less concave
Celloidin
47
Embedding medium for Plane-Concave knives that are more concave
Paraffin
48
Embedding Medium for Biconcave and Plane-Wedge knives
Paraffin
49
Microtomes that use plane-concave knives that are less concave
Sliding Microtome
50
Microtomes that use plane-concave knives that are more concave
Base Sledge or Rotary Microtome
51
Microtomes that use biconcave knives
Rotary Microtome
52
Microtomes that use plane-wedge knives
Base-Sledge Microtome
53
Microtome knife that is coated with polytetrafluroethylene
Disposable Blade
54
Microtome knife that is for partially calcified materials, paraffin, and frozen sections
Safety Razor Blade
55
Bevel Angle
27 to 32 degrees
56
Cutting Angle
14 degrees
57
Most important knife angle
Clearing Angle
58
Clearing Angle
5 to 10 degrees
59
Heel to Toe sharpening
Honing
60
Toe to Heel
Stropping
61
Honing vs Stropping: Removing of Gross nicks
Honing
62
Honing vs Stropping: Removal of Burr/Irregularities
Stropping
63
Sharpening method that aims to acquire an even edge
Honing
64
Final polishing of the knife edge
Stropping
65
Most common Hone
Belgium Yellow
66
Hone that has a more polishing effect
Arkansas
67
Hone used for badly nicked knives
Fine Carborundum
68
Lubricants for Honing
Soapy water Mineral Oil Clove Oil Xylene Liquid Paraffin
69
Knife Sharpener used for grinding and removing nicks
Flat Glass plate (w/ finely powdered aluminum oxide)
70
Knife Sharpener for final polishing
Diamantine
71
In Stropping, instead of hones, what is used?
Paddle Strop
72
Enumerate 5 adhesives
Mayer's Egg Albumin Dried Albumin Starch Paste Sodium Silicate Resin
73
Most common adhesive
Mayer's Egg Albumin
74
Main Adhesive in Mayer's Egg Albumin
Egg white
75
Increases viscosity and prevents drying in Mayer's Egg Albumin
Glycerol
76
Prevents mold formation in Mayer's Egg Albumin
Thymol Cyrstals
77
Floatation water bath temp
45 to 50 C (5-10C below melting point)
78
Inside of Floatation bath is colored using what
Enamel Black
79
Recommend number of tissue sections for Floatation
3 to 5
80
Slide Dryer temp
5 to 10 C above melting point
81
Used to store the mounted section during the drying
Metal Racks (w/ 25-slide divisions)

HistoPath Lec 2ndYR 2ndSem Midterms (5 decks)

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