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HistoPath Lec 2ndYR 2ndSem Midterms > Impregnation and Embedding > Flashcards

Impregnation and Embedding Flashcards

1
Q

Process that removes the clearing agent and fills tissue cavities

A

Impregnation

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2
Q

Tissue air holes are caused by what

A

Incomplete Impregnation

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3
Q

Most common paraffin wax melting point

A

58 to 56 Celcius

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4
Q

Paraffin Wax temperature that causes brittleness

A

> 60 C

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5
Q

Paraffin oven must be maintained at what temperature

A

2-5 C above melting point of wax

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6
Q

Paraffine wax is not recommended for what type of tissues

A

fatty tissues

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7
Q

3 ways of Paraffin Wax Impregnation

A

Manual Processing
Automatic Processing
Vacuum Embedding

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8
Q

How many changes of paraffin are in Manual Processing

A

3

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9
Q

Interval of beaker change in Manual Processing

A

1hr

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10
Q

An automatic processor has how many stations

A

12

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11
Q

Which stations of the Automatic Processor does infiltration take place

A

11 and 12

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12
Q

Vastest type of Paraffin Wax Impregnation

A

Vacuum Embedding

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13
Q

Substitutes for Paraffine Wax

A

Paraplast
Embeddol
Bioloid
Ester Wax
Water Soluble Waxes

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14
Q

Melting Point of paraplast

A

56 to 57 C

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15
Q

Mixture of Pure paraffin and synthetic plastic polymer

A

paraplast

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16
Q

Melting point of Embeddol

A

56 to 57 C

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17
Q

A semisynthetic substitute to Paraffin Wax and is used for embedding dyes

A

Bioloid

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18
Q

Melting Point of Ester Wax

A

46 to 48 C

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19
Q

Melting Point of Water Soluble Waxes

A

38 to 42 C or 45 to 56 C

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20
Q

Most common water soluble wax

A

Carbowax

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21
Q

Paraffine Wax substitute for Urgent processing

A

Carbowax

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22
Q

Purified form of nitrocellulose/gun cotton

A

Celloidin a.k.a Colloidin

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23
Q

Type of impregnation recommended for neurological tissues

A

Celloidin Impregnation

24
Q

Type of impregnation for specimens with large and hollow cavities which tend to collapse as well as for hard and dense tissues

A

Celloidin Impregnation

25
Q

What are the percentages for the increasing concentration of Celloidin Infiltration

A

2% (Thin celloidin)
4% (Medium celloidin)
8% (Thick celloidin)

26
Q

Wet tissues blocks are placed in?

A

70% - 80% Alcohol

27
Q

Dry tissues blocks are placed in?

A

Gilson’s Mixture (Chloroform & Cedarwood Oil)

28
Q

Examples of samples for wet tissue blocks

A

Bones
Brain
Teeth

29
Q

Example of samples for dry tissue blocks

A

Whole eye sections

30
Q

Explosive when dry

A

Nitrocellulose

31
Q

Needed to prevent tissue racking in chrome mordanted tissues

A

Plasticizer

32
Q

Type of Impregnation for histochemical, enzyme studies, and frozen sections

A

Gelatin Impregnation

33
Q

Tissue size for Gelatin Impregnation

A

less than 2-3mm thick

34
Q

Other names for Embedding

A

Casting
Blocking
Molding

35
Q

Arrangement of the tissue in a precise position in the mold during embedding

A

Orientation

36
Q

Proper orientation in embedding

A

Surface should be parallel to the bottom of the mold

37
Q

Orientation:

All layers in transverse sections

A

Tubular Tissue

38
Q

Orientation:

All layers should come (pahiga)

A

Skin and Intestine

39
Q

Orientation:

Keep in Center

A

Endometrial Curetting

40
Q

Orientation:

Keep diagonally

A

Long Tissue

41
Q

Orientation:

Swiss Roll

A

Membrane

42
Q

Types of Molds

A

Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings
Disposable Embedding Molds

43
Q

Type of Molds:

Two L-shaped metal plates, strips of heavy brass metal

A

Leuckhart’s Embedding Mold

44
Q

Types of Molds:

Used for batch embedding

A

Compound Embedding Unit

45
Q

Examples of materials used in Plastic Embedding Rings

A

Silicon
Metal

46
Q

In Plastic Ice Trays, it should first be greased with what before process of embedding

A

Glycerin
Liquid Paraffin Wax

47
Q

3 Embedding Modes

A

Celloidin/Nitrocellulose
Double-Embedding
Plastic Resin

48
Q

Embedding Methods:

First infiltrated with Celloidin and subsequently embedded in a paraffin wax

A

Double-Embedding

49
Q

Embedding Methods:

For high resolution light microscopy of thinner than usual sections, renal biopsies, and bone marrow biopsies

A

Plastic Resin Embedding

50
Q

Plastic Resin Embedding Media

A

Epoxy
Polyester
Acrylic Plastic

51
Q

Plastic Resin Embedding:

Most widely applied medium

A

Epoxy

52
Q

3 examples of Epoxy

A

Bisphenol A (Araldite)
Glycerol (Epon)
Cyclohexene Dioxide (Spurr)

53
Q

Plastic Resin Embedding:

For high resolution light microscopy

A

Acrylic plastics

54
Q

Examples of Acrylic Plastics

A

Polyglycol Methacrylate (GMA)
Methyl Methacrylate (MMA)

55
Q

Catalyst for Acrylic Plastics

A

Benzoyl Peroxide

56
Q

3 types of general tissue impregnation

A

Paraffin Wax Impregnation
Celloidin Impregnation
Gelatin Impregnation

HistoPath Lec 2ndYR 2ndSem Midterms (5 decks)

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