Impregnation and Embedding

Question 1

Process that removes the clearing agent and fills tissue cavities

Answer 1

Impregnation

Question 2

Tissue air holes are caused by what

Answer 2

Incomplete Impregnation

Question 3

Most common paraffin wax melting point

Answer 3

58 to 56 Celcius

Question 4

Paraffin Wax temperature that causes brittleness

Answer 4

> 60 C

Question 5

Paraffin oven must be maintained at what temperature

Answer 5

2-5 C above melting point of wax

Question 6

Paraffine wax is not recommended for what type of tissues

Answer 6

fatty tissues

Question 7

3 ways of Paraffin Wax Impregnation

Answer 7

Manual Processing
Automatic Processing
Vacuum Embedding

Question 8

How many changes of paraffin are in Manual Processing

Answer 8

3

Question 9

Interval of beaker change in Manual Processing

Answer 9

1hr

Question 10

An automatic processor has how many stations

Answer 10

12

Question 11

Which stations of the Automatic Processor does infiltration take place

Answer 11

11 and 12

Question 12

Vastest type of Paraffin Wax Impregnation

Answer 12

Vacuum Embedding

Question 13

Substitutes for Paraffine Wax

Answer 13

Paraplast
Embeddol
Bioloid
Ester Wax
Water Soluble Waxes

Question 14

Melting Point of paraplast

Answer 14

56 to 57 C

Question 15

Mixture of Pure paraffin and synthetic plastic polymer

Answer 15

paraplast

Question 16

Melting point of Embeddol

Answer 16

56 to 57 C

Question 17

A semisynthetic substitute to Paraffin Wax and is used for embedding dyes

Answer 17

Bioloid

Question 18

Melting Point of Ester Wax

Answer 18

46 to 48 C

Question 19

Melting Point of Water Soluble Waxes

Answer 19

38 to 42 C or 45 to 56 C

Question 20

Most common water soluble wax

Answer 20

Carbowax

Question 21

Paraffine Wax substitute for Urgent processing

Answer 21

Carbowax

Question 22

Purified form of nitrocellulose/gun cotton

Answer 22

Celloidin a.k.a Colloidin

Question 23

Type of impregnation recommended for neurological tissues

Answer 23

Celloidin Impregnation

Question 24

Type of impregnation for specimens with large and hollow cavities which tend to collapse as well as for hard and dense tissues

Answer 24

Celloidin Impregnation

Question 25

What are the percentages for the increasing concentration of Celloidin Infiltration

Answer 25

2% (Thin celloidin)
4% (Medium celloidin)
8% (Thick celloidin)

Question 26

Wet tissues blocks are placed in?

Answer 26

70% - 80% Alcohol

Question 27

Dry tissues blocks are placed in?

Answer 27

Gilson’s Mixture (Chloroform & Cedarwood Oil)

Question 28

Examples of samples for wet tissue blocks

Answer 28

Bones
Brain
Teeth

Question 29

Example of samples for dry tissue blocks

Answer 29

Whole eye sections

Question 30

Explosive when dry

Answer 30

Nitrocellulose

Question 31

Needed to prevent tissue racking in chrome mordanted tissues

Answer 31

Plasticizer

Question 32

Type of Impregnation for histochemical, enzyme studies, and frozen sections

Answer 32

Gelatin Impregnation

Question 33

Tissue size for Gelatin Impregnation

Answer 33

less than 2-3mm thick

Question 34

Other names for Embedding

Answer 34

Casting
Blocking
Molding

Question 35

Arrangement of the tissue in a precise position in the mold during embedding

Answer 35

Orientation

Question 36

Proper orientation in embedding

Answer 36

Surface should be parallel to the bottom of the mold

Question 37

Orientation:

All layers in transverse sections

Answer 37

Tubular Tissue

Question 38

Orientation:

All layers should come (pahiga)

Answer 38

Skin and Intestine

Question 39

Orientation:

Keep in Center

Answer 39

Endometrial Curetting

Question 40

Orientation:

Keep diagonally

Answer 40

Long Tissue

Question 41

Orientation:

Swiss Roll

Answer 41

Membrane

Question 42

Types of Molds

Answer 42

Leuckhart’s Embedding Mold
Compound Embedding Unit
Plastic Embedding Rings
Disposable Embedding Molds

Question 43

Type of Molds:

Two L-shaped metal plates, strips of heavy brass metal

Answer 43

Leuckhart’s Embedding Mold

Question 44

Types of Molds:

Used for batch embedding

Answer 44

Compound Embedding Unit

Question 45

Examples of materials used in Plastic Embedding Rings

Answer 45

Silicon
Metal

Question 46

In Plastic Ice Trays, it should first be greased with what before process of embedding

Answer 46

Glycerin
Liquid Paraffin Wax

Question 47

3 Embedding Modes

Answer 47

Celloidin/Nitrocellulose
Double-Embedding
Plastic Resin

Question 48

Embedding Methods:

First infiltrated with Celloidin and subsequently embedded in a paraffin wax

Answer 48

Double-Embedding

Question 49

Embedding Methods:

For high resolution light microscopy of thinner than usual sections, renal biopsies, and bone marrow biopsies

Answer 49

Plastic Resin Embedding

Question 50

Plastic Resin Embedding Media

Answer 50

Epoxy
Polyester
Acrylic Plastic

Question 51

Plastic Resin Embedding:

Most widely applied medium

Answer 51

Epoxy

Question 52

3 examples of Epoxy

Answer 52

Bisphenol A (Araldite)
Glycerol (Epon)
Cyclohexene Dioxide (Spurr)

Question 53

Plastic Resin Embedding:

For high resolution light microscopy

Answer 53

Acrylic plastics

Question 54

Examples of Acrylic Plastics

Answer 54

Polyglycol Methacrylate (GMA)
Methyl Methacrylate (MMA)

Question 55

Catalyst for Acrylic Plastics

Answer 55

Benzoyl Peroxide

Question 56

3 types of general tissue impregnation

Answer 56

Paraffin Wax Impregnation
Celloidin Impregnation
Gelatin Impregnation