Impregnation and Embedding Flashcards

1
Q

Process that removes the clearing agent and fills tissue cavities

A

Impregnation

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2
Q

Tissue air holes are caused by what

A

Incomplete Impregnation

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3
Q

Most common paraffin wax melting point

A

58 to 56 Celcius

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4
Q

Paraffin Wax temperature that causes brittleness

A

> 60 C

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5
Q

Paraffin oven must be maintained at what temperature

A

2-5 C above melting point of wax

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6
Q

Paraffine wax is not recommended for what type of tissues

A

fatty tissues

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7
Q

3 ways of Paraffin Wax Impregnation

A

Manual Processing
Automatic Processing
Vacuum Embedding

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8
Q

How many changes of paraffin are in Manual Processing

A

3

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9
Q

Interval of beaker change in Manual Processing

A

1hr

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10
Q

An automatic processor has how many stations

A

12

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11
Q

Which stations of the Automatic Processor does infiltration take place

A

11 and 12

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12
Q

Vastest type of Paraffin Wax Impregnation

A

Vacuum Embedding

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13
Q

Substitutes for Paraffine Wax

A

Paraplast
Embeddol
Bioloid
Ester Wax
Water Soluble Waxes

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14
Q

Melting Point of paraplast

A

56 to 57 C

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15
Q

Mixture of Pure paraffin and synthetic plastic polymer

A

paraplast

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16
Q

Melting point of Embeddol

A

56 to 57 C

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17
Q

A semisynthetic substitute to Paraffin Wax and is used for embedding dyes

A

Bioloid

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18
Q

Melting Point of Ester Wax

A

46 to 48 C

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19
Q

Melting Point of Water Soluble Waxes

A

38 to 42 C or 45 to 56 C

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20
Q

Most common water soluble wax

A

Carbowax

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21
Q

Paraffine Wax substitute for Urgent processing

A

Carbowax

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22
Q

Purified form of nitrocellulose/gun cotton

A

Celloidin a.k.a Colloidin

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23
Q

Type of impregnation recommended for neurological tissues

A

Celloidin Impregnation

24
Q

Type of impregnation for specimens with large and hollow cavities which tend to collapse as well as for hard and dense tissues

A

Celloidin Impregnation

25
What are the percentages for the increasing concentration of Celloidin Infiltration
2% (Thin celloidin) 4% (Medium celloidin) 8% (Thick celloidin)
26
Wet tissues blocks are placed in?
70% - 80% Alcohol
27
Dry tissues blocks are placed in?
Gilson's Mixture (Chloroform & Cedarwood Oil)
28
Examples of samples for wet tissue blocks
Bones Brain Teeth
29
Example of samples for dry tissue blocks
Whole eye sections
30
Explosive when dry
Nitrocellulose
31
Needed to prevent tissue racking in chrome mordanted tissues
Plasticizer
32
Type of Impregnation for histochemical, enzyme studies, and frozen sections
Gelatin Impregnation
33
Tissue size for Gelatin Impregnation
less than 2-3mm thick
34
Other names for Embedding
Casting Blocking Molding
35
Arrangement of the tissue in a precise position in the mold during embedding
Orientation
36
Proper orientation in embedding
Surface should be parallel to the bottom of the mold
37
Orientation: All layers in transverse sections
Tubular Tissue
38
Orientation: All layers should come (pahiga)
Skin and Intestine
39
Orientation: Keep in Center
Endometrial Curetting
40
Orientation: Keep diagonally
Long Tissue
41
Orientation: Swiss Roll
Membrane
42
Types of Molds
Leuckhart's Embedding Mold Compound Embedding Unit Plastic Embedding Rings Disposable Embedding Molds
43
Type of Molds: Two L-shaped metal plates, strips of heavy brass metal
Leuckhart's Embedding Mold
44
Types of Molds: Used for batch embedding
Compound Embedding Unit
45
Examples of materials used in Plastic Embedding Rings
Silicon Metal
46
In Plastic Ice Trays, it should first be greased with what before process of embedding
Glycerin Liquid Paraffin Wax
47
3 Embedding Modes
Celloidin/Nitrocellulose Double-Embedding Plastic Resin
48
Embedding Methods: First infiltrated with Celloidin and subsequently embedded in a paraffin wax
Double-Embedding
49
Embedding Methods: For high resolution light microscopy of thinner than usual sections, renal biopsies, and bone marrow biopsies
Plastic Resin Embedding
50
Plastic Resin Embedding Media
Epoxy Polyester Acrylic Plastic
51
Plastic Resin Embedding: Most widely applied medium
Epoxy
52
3 examples of Epoxy
Bisphenol A (Araldite) Glycerol (Epon) Cyclohexene Dioxide (Spurr)
53
Plastic Resin Embedding: For high resolution light microscopy
Acrylic plastics
54
Examples of Acrylic Plastics
Polyglycol Methacrylate (GMA) Methyl Methacrylate (MMA)
55
Catalyst for Acrylic Plastics
Benzoyl Peroxide
56
3 types of general tissue impregnation
Paraffin Wax Impregnation Celloidin Impregnation Gelatin Impregnation