Clearing Flashcards
Main purpose of Clearing
Create a bridge between dehydration and infiltration
Characteristics of Good Clearing Agent
Miscible with Alcohol
Miscible with Paraffin Wax
Does not produce tissue shrinkage
Make tissues transparent
Should not dissolve aniline dyes
Should not evaporate quickly in water bath
Clearing time for Xylene
30mins to 1hr
Clearing time for Toluene
1hr to 2hrs
Clearing time for Benzene
15mins to 1hr
Fastest clearing agent
Benzene
May lead to aplastic anemia
Benzene
Clearing agent for Tough tissues and Large specimens
Chloroform
Clearing time for Chloroform
6 to 24hrs
Clearing agent for smooth muscles and CNS tissues
Cedarwood Oil
Clearing time for Cedarwood Oil
2 to 3 days
Clearing agent of insects, embryos, and delicate tissues
Aniline Oil
Clearing agent that is similar to Chloroform but cheaper
Carbon Tetrachloride
Clearing agent that is considered superior among other agents since it can perform both dehydration and clearing processes
Tetrahydrofuran
Clearing agent used when directly from water such as frozen sections
Gum Syrup and Glycerin
Clearing agent for Smooth muscle and Skin
Oil of Bergamot
Clearing agent for Skin
Oil of Oreganum
Clearing agent which is artificial and is for delicate tissues
Oil of Wintergreen
Clearing agent that has a foul odor and is used for smooth muscles
Carbon Disulfide
Clearing agent for Friable tissues
Carbol xylene
Clearing agent for delicate tissues like eyes
Terpineol
Clearing agent for Smooth Muscles
Phenol
