Microscopy, Staining, And Classification Flashcards

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0
Q

What were the old five basic kingdoms?

A

Animalia, plantae, fungi, protista, prokaryotae

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1
Q

What is the proper way to write binomial nomenclature?

A

Genus’ first letter capitalized and everything italicized (species too)

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2
Q

Who proposed the three domains of eukarya, bacteria, and archae by comparing nucleotide sequences of rRNA subunits?

A

Carl Woese

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3
Q

What are the new divisions of the eukarya domain?

A

Animals, plants, fungi, protozoans, algae

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4
Q

What is resolution?

A

The shortest distance between two points on a specimen that can still be distinguished by the observer as separate entities

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5
Q

What is contrast?

A

Differences in intensity between two objects or between an object and background

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6
Q

What is contrast important for?

A

Determining resolution

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7
Q

What process in microbiology is important for increasing contrast?

A

Staining

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8
Q

Using stains and dyes in microbiology, what two concepts are enhanced?

A

Resolution and contrast

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9
Q

What is the name of the purple simple stain?

A

Crystal violet

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10
Q

What is the name of the magenta simple stain?

A

Safranin

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11
Q

What is the name of the blue simple stain?

A

Methylene blue

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12
Q

What is the name of the green simple stain?

A

Malachite green

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13
Q

What is the name of the red simple stain?

A

Carbol fuchsin

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14
Q

What is the name of the purple and magenta differential stains?

A

Gram stain

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15
Q

What is the name of the red and blue differential stain?

A

Acid-fast stain

16
Q

What is the name of the green and magenta differential stain?

A

Endospore stain

17
Q

What is the result of a gram stain?

A

Purple gram + cells and magenta gram - cells

18
Q

What type of bacteria is referred to as “acid-fast bacteria”?

A

Mycobacteria

19
Q

What are the results of the acid-fast stain?

A

Red acid-fast cells and blue non-acid-fast cells

20
Q

What would happen if you did a gram stain on mycobacteria?

A

Nothing would show up

21
Q

Are human cells and tissues acid-fast cells?

A

Noooooooo

22
Q

What are the results of the endospore stain?

A

Green endospores and magenta vegetative cells

23
Q

What are dichotomous keys?

A

A series of paired statements that enable a choice to be made about two things to determine what organism is present

24
Q

What type of disease is necrotizing fasciitis?

A

Emerging

25
Q

What is another name for necrotizing fasciitis?

A

Flesh-eating disease

26
Q

What is the most common cause of necrotizing fasciitis?

A

Streptococcus pyogenes (gram + bacteria)