Microscopy, Staining, And Classification Flashcards
What were the old five basic kingdoms?
Animalia, plantae, fungi, protista, prokaryotae
What is the proper way to write binomial nomenclature?
Genus’ first letter capitalized and everything italicized (species too)
Who proposed the three domains of eukarya, bacteria, and archae by comparing nucleotide sequences of rRNA subunits?
Carl Woese
What are the new divisions of the eukarya domain?
Animals, plants, fungi, protozoans, algae
What is resolution?
The shortest distance between two points on a specimen that can still be distinguished by the observer as separate entities
What is contrast?
Differences in intensity between two objects or between an object and background
What is contrast important for?
Determining resolution
What process in microbiology is important for increasing contrast?
Staining
Using stains and dyes in microbiology, what two concepts are enhanced?
Resolution and contrast
What is the name of the purple simple stain?
Crystal violet
What is the name of the magenta simple stain?
Safranin
What is the name of the blue simple stain?
Methylene blue
What is the name of the green simple stain?
Malachite green
What is the name of the red simple stain?
Carbol fuchsin
What is the name of the purple and magenta differential stains?
Gram stain
What is the name of the red and blue differential stain?
Acid-fast stain
What is the name of the green and magenta differential stain?
Endospore stain
What is the result of a gram stain?
Purple gram + cells and magenta gram - cells
What type of bacteria is referred to as “acid-fast bacteria”?
Mycobacteria
What are the results of the acid-fast stain?
Red acid-fast cells and blue non-acid-fast cells
What would happen if you did a gram stain on mycobacteria?
Nothing would show up
Are human cells and tissues acid-fast cells?
Noooooooo
What are the results of the endospore stain?
Green endospores and magenta vegetative cells
What are dichotomous keys?
A series of paired statements that enable a choice to be made about two things to determine what organism is present
What type of disease is necrotizing fasciitis?
Emerging
What is another name for necrotizing fasciitis?
Flesh-eating disease
What is the most common cause of necrotizing fasciitis?
Streptococcus pyogenes (gram + bacteria)
