microscopy DEFINITELY IN EXAM Flashcards

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1
Q

what are the negatives of light mcroscopes

A
  • limited magnification
    (cannot easily view structures inside of the cell)
  • limited resolution
    (the image is blurred so cannot see fine details)
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2
Q

what are the advantages of electron microscopes

A
  • greater magnification

- greater resolution

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3
Q

what is the formula for magnification

A

magnification =
size of image
————————-
size of real object

(IAM triangle)

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4
Q

what do the coarse and fine focusing knobs do

A

bring the image into focus by moving the stage up or down

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5
Q

ways to ensure an accurate and scientific diagram of cells viewed in the sample on a microscope

A
  • use a pencil with a sharp point
  • use clear unbroken lines to draw the structures she can see
  • drawing should take up at least half of the space available
  • include a title
  • draw subcellular structures in proportion
  • label the important features of her diagram with straight uncrossed lines
  • write the magnification it was observed under
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