microscopy DEFINITELY IN EXAM

Question 1

what are the negatives of light mcroscopes

Answer 1

• limited magnification
  (cannot easily view structures inside of the cell)
• limited resolution
  (the image is blurred so cannot see fine details)

Question 2

what are the advantages of electron microscopes

Answer 2

• greater magnification

- greater resolution

Question 3

what is the formula for magnification

Answer 3

magnification =
size of image
————————-
size of real object

(IAM triangle)

Question 4

what do the coarse and fine focusing knobs do

Answer 4

bring the image into focus by moving the stage up or down

Question 5

ways to ensure an accurate and scientific diagram of cells viewed in the sample on a microscope

Answer 5

• use a pencil with a sharp point
• use clear unbroken lines to draw the structures she can see
• drawing should take up at least half of the space available
• include a title
• draw subcellular structures in proportion
• label the important features of her diagram with straight uncrossed lines
• write the magnification it was observed under