Microscopy

Question 1

Label a microscope (do it in your head. sorry)

Answer 1

ocular lens
body tube
nose piece
objective lenses
arm
base
light source
diaphragm
stage
stage clips
fine & coarse adjustment knobs

Question 2

What is the magnification of the ocular lens?

Answer 2

10X

Question 3

What are the magnification of objective lenses?

Answer 3

4X, 10X, 40X, 100X

Question 4

What is magnification?

Answer 4

how much larger the object appears to its real size

Question 5

What is a pro & con of magnification?

Answer 5

can see image in more detail

see less of image

Question 6

What is resolution?

Answer 6

ability to form seperate images of objects that are close together

resolving power, therefore, the minimum distance two points can be separated and still be distinguished as seperate

Smaller resolving power the better resolution

Question 7

What can you tell me about light microscopes?

Answer 7

inexpensive
easy to use
used to study stained or living cells
IN COLOUR
can magnify objects up to 2000X
can resolve objects 200 nm apart

Question 8

What can you tell me about electron microscopes?

Answer 8

can magnify up to 250,000X
can resolve objects 0.2nm apart
requires dead and chemically treated cells
NO COLOUR

Question 9

What are some relative sizes?

Answer 9

cells  - 100um
organelles - 10um
bacteria - 1um
viruses - 100nm
cell membrane thickness - 10nm
molecule - 1nm

Question 10

What part of cells cannot be seen under light microscope?

Answer 10

ribosome
endoplasmic reticulum
golgi apparatus

cytoskeleton

Question 11

Tell me the basic microscope math

Answer 11

FOV lower power X magnification lower power

= FOV higher power X magnification higher power

Question 12

Tell me another basic microscope math

Answer 12

size of object = FOV/# of objects in field

Question 13

What is the difference between object and image?

Answer 13

object is one specific thing in FOV

image is all objects and space in between them in FOV

Question 14

How do I calculate magnification using scale bar?

Answer 14

1 - measure scale bar (mm)
2 - convert mm to um (not actually a u, more like upside down h)
3 - divide image scale bar by length of of actual object (no units)
4 - multiply scale bar label by last answer (um (upside down h))

Question 15

What is the magnification formula?

Answer 15

length of object / actual size (at the atomic level)