Microscopy

Question 1

Whats microscopy used to do?

Answer 1

Study very small objects

To look at cells or organelles

Question 2

What do light microscopes use

Answer 2

An external light source

Question 3

Light microscope negatives

Answer 3

Lower magnification
Lower resolution
Difficulty distinguishing
Does not zoom in as much

Question 4

Light microscope advantages

Answer 4

Cheaper

Can see whole cells nuclei and vacuoles

Question 5

What do electron microscopes use?

Answer 5

Electrons to generate the images

Question 6

Electron microscope advantages

Answer 6

Magnification much higher
Resolution much higher
Used for much smaller things like ribosomes

Question 7

Disadvantage of electron microscopes

Answer 7

Expensive

Question 8

How to prepare a light microscope

Answer 8

1) take a slide and put a small water droplet on it
2) take a thin layer of your specimen so the light goes through
3) use a stain (a chemical that you put on the specimen) this highlights specific organelles
4) place a cover slip on top and avoid bubbles

Question 9

How to calculate magnification

Answer 9

Image size= magnification x actual size