Microscopy Flashcards

0
Q

Base

A

Supports microscope; held in transport

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1
Q

Arm

A

Connects the base and barrel; held in transport

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2
Q

Body tube

A

Holds revolving nosepiece; houses lenses and mirrors

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3
Q

Condenser

A

Focus light on specimen

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4
Q

Course focus adjustment

A

Moves stage a lot; only used in low power; raises and lowers stage

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5
Q

Diaphragm

A

Regulates the light (located on bottom if stage)

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6
Q

Fine focus adjustment

A

Small movements of stage- up and down

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7
Q

High power objective (40x)

A

Magnifies

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8
Q

Iris diaphragm lever

A

Beneath condenser- further adjusts the amount of light a specimen receives

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9
Q

Light source

A

Provides light for viewing specimen

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10
Q

Low power objective (10x)

A

Magnifies- medium power

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11
Q

Mechanical stage

A

Holds slides

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12
Q

Mechanical stage adjuster

A

Top- forward and back

Bottom- stage clip side to side

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13
Q

Nose piece (rotating turret, revolving nosepiece)

A

Holds objectives; rotates to change objective

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14
Q

Ocular lens

A

Eye piece- where you look; usually 10x magnification

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15
Q

Oil immersion (100x)

A

Magnifies; not used in a&p 1

16
Q

Scanning objective (4x)

A

Low power; red band; end position

17
Q

Slide retainer clip (stage clip)

A

Holds slides in place

18
Q

Depth of field

A

Thickness if the plane of focus; how much the specimen can move and still be in focus

19
Q

Magnification/ magnifying power

A

Amount the specimen has been enlarged in appearance, not actual size

20
Q

Parfocal

A

Stay in focus when magnification is changed; objectives can be changed with little to no refocusing

21
Q

Resolution

A

Ability to distinguish 2 distinct points; higher resolution- closer the points can be while still able to distinguish them

22
Q

Total magnification

A

Ocular lens and objective (10x times 4x is 40x magnification)

23
Q

Working distance

A

Decreases as magnification and numerical aperture both increase; distance between the front edge of the objective lens and the specimen surface when the specimen is focused

24
Q

Field of view

A

The amount of things you are looking for (as magnification goes up, field of view goes down)