Microscopy Flashcards
Base
Supports microscope; held in transport
Arm
Connects the base and barrel; held in transport
Body tube
Holds revolving nosepiece; houses lenses and mirrors
Condenser
Focus light on specimen
Course focus adjustment
Moves stage a lot; only used in low power; raises and lowers stage
Diaphragm
Regulates the light (located on bottom if stage)
Fine focus adjustment
Small movements of stage- up and down
High power objective (40x)
Magnifies
Iris diaphragm lever
Beneath condenser- further adjusts the amount of light a specimen receives
Light source
Provides light for viewing specimen
Low power objective (10x)
Magnifies- medium power
Mechanical stage
Holds slides
Mechanical stage adjuster
Top- forward and back
Bottom- stage clip side to side
Nose piece (rotating turret, revolving nosepiece)
Holds objectives; rotates to change objective
Ocular lens
Eye piece- where you look; usually 10x magnification
Oil immersion (100x)
Magnifies; not used in a&p 1
Scanning objective (4x)
Low power; red band; end position
Slide retainer clip (stage clip)
Holds slides in place
Depth of field
Thickness if the plane of focus; how much the specimen can move and still be in focus
Magnification/ magnifying power
Amount the specimen has been enlarged in appearance, not actual size
Parfocal
Stay in focus when magnification is changed; objectives can be changed with little to no refocusing
Resolution
Ability to distinguish 2 distinct points; higher resolution- closer the points can be while still able to distinguish them
Total magnification
Ocular lens and objective (10x times 4x is 40x magnification)
Working distance
Decreases as magnification and numerical aperture both increase; distance between the front edge of the objective lens and the specimen surface when the specimen is focused
