Microscopy Flashcards

0
Q

A microscope

A

An instrument used to see objects too small to be seen with the naked eye

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1
Q

Role of Jansen and his son in developing the microscope (1590)

A
  • Lenses placed in a tube
  • Forerunner of the compound microscope
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2
Q

Role of Hooke in microscopy (1665)

A
  • Observed cork compartments
  • He named them ‘cells’
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3
Q

Role of van Leeuwenhoek in microscopy (1674)

A
  • Simple microscopes with improved lenses (270x)
  • Studied blood, yeast, insects etc.
  • First to describe bacteria
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4
Q

Role of Schwann, Schleiden and Virchow in developing microscopy (1839)

(Grade 10 only)

A

Formulated the cell theory

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5
Q

Cell theory

(Grade 10 only)

A
  • All living organisms are made up of cells
  • the cell is the basic and smallest unit of life
  • All cells develop from already existing cells

Modern additions:

  • Energy flows inside cells
  • DNA is passed on from cell to cell and instructs all cell activity
  • All cells have the same basic chemical composition
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6
Q

Maximum magnification of a light microscope

A

1250-1500x

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7
Q

Role of Knott and Ruska in microscopy (1931)

A
  • Developed the first electron microscope
  • these can today magnify up to about 1 000 000x
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8
Q

Microscope tube

A

Part in which the eyepiece fits

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9
Q

Microscope eyepiece

A

The upper lens of the microscope for magnification (usually 10x)

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10
Q

Microscope objective

A
  • Lower lens that is movable
  • Objects can be studied with different magnifications
  • e.g. 4X, 10X and 40X
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11
Q

Microscope nose piece

A

Part of the microscope that carries the objectives

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12
Q

Microscope clip

A

Keeps slides in position on the stage

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13
Q

Microscope arm

A

Handle of the microscope

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14
Q

Microscope coarse adjustment knob

A
  • Moves the stage/tube with large movements to bring the object into focus
  • Only used with the low power objective lens
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15
Q

Microscope fine adjustment knob

A

Moves the tube/stage with fine movements for final focus

16
Q

Microscope light source

A
  • Provides light that shines through the opening as well as the mounted object to the eye
  • Can be electric or from the sun using a mirror
17
Q

Microscope mirror

A

In some microscopes it reflects light from the sun

18
Q

Microscope condenser

A

Concentrates rays of light from the light source onto the object

19
Q

Microscope diaphragm

A
  • Regulates the amount of light that shines onto the object
  • Can open and close like the eyes iris
20
Q

How to calculate total magnification

A

Eye piece magnification X the objective magnification

E.g. 10 X 40 = 400x

21
Q

Some ways to take care of a microscope

A
  • Hold it by the arm and base when carrying
  • Never touch lenses
  • Always start with smallest objective lens
  • Cover the microscope to protect from dust
  • Place on a firm, dry surface
22
Q

Preparing a wet mount slide

A
  • Place the specimen on the slide with a drop of water
  • cover it with a coverslip
  • Draw stain under the coverslip using tissue on the other side of the coverslip
  • Dry the surface of the slide
23
Q

Using a microscope

A
  1. Turn on light
  2. Set lowest power objective
  3. Place slide on stage
  4. Centre the specimen in the light
  5. Focus using coarse adjustment
  6. Adjust light using diaphragm and condenser to sharpest
  7. Move to a larger lens
  8. Adjust focus using the fine adjustment only
25
Q

Formula to calculate actual size of specimen

(Grade 10 only)

A
26
Q

Formula to calculate magnification using a scale bar

(Grade 10 only)

A