microscopy

Question 1

Magnification formula

Answer 1

I/AxM
image size/ actual size X magnification

Question 2

what can you use light microscopes for?

Answer 2

individual cells
large subcellular structures (eg nuclei)

Question 3

what can you use electron microscopes for?

Answer 3

smaller subcellular structures in more detail(ribosomes, chloroplasts ect)

Question 4

pros and cons of light microscopes

Answer 4

pros- cheaper , easy to use, light weight, can use a living cell
cons- lower resolution(image not as sharp), lower magnification(not as detailed image)

Question 5

pros and cons of electron microscopes

Answer 5

pros- higher resolution(sharper image), higher magnification(clearer more detailed image)
cons- Expensive, can only use dead cells, higher maintenance, take up lots of space and hard to access

Question 6

how to get from micrometres(ym) to millimetres(mm)

Answer 6

divide by 1000

Question 7

Parts of a microscope

Answer 7

eyepiece
coarse adjustment knob
fine adjustment knob
light
objective lenses
stage
clips

Question 8

onion slide practical

Answer 8

1- drop of water on clean slide
2- cut up onion into layers and use tweezers to peel off epidermal tissue layer
3- place on slide and apply a drop of iodine
4- place a clear cover slip on top of onion ensuring no air bubbles
5- clip slide onto stage
6- use coarse adjustment knob to move stage up and down
7- use coarse adjustment knob until view is focused
8- focus properly with fine adjustment knob