culturing microorganisms Flashcards
what is the jelly called you culture bacteria on
Agar plate
how to culture bacteria practical
1- keep lid over agar plate to avoid contamination
2-flame inoculating spreader to kill any bacteria
3-flame neck of the tube of bacteria to prevent any contaminating microbes entering
4-use the sterile spreader to spread the bacteria over the plate evenly
5-use tweezers to place the soaked paper disks covered in different antibiotics onto the plate
6-secure lid with tape and store the dish upside down
what will you see if the antibiotic worked
a ring of where bacteria have died- zone of inhibition
what is the school limit for bacteria growing temp and why
25 degrees as it prevents harmful bacteria being grown that would harm a human at 37 degrees
how to calculate zone of inhibition size
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