microscopy

Question 1

characteristics of a light (optical) microscope

Answer 1

uses light to form an image
low resolution
cant see RER, lysosomes etc
200nm max resolution

Question 2

characteristics of a scanning electron microscope

Answer 2

uses electrons
max resolution of 0.02nm
observe small organelles
can be used on thick specimens

Question 3

characteristics of a transmission electron microscope

Answer 3

allows internal structures to be seen
only use very thin specimens
lengthy treatment to prepare specimens

Question 4

magnification=

Answer 4

image over real

size of real object

Question 5

how to prepare cells for cell fractionation

Answer 5

make sure they are cold and buffered, and have the same water potential as the solution.

Question 6

describe homogenation

Answer 6

cells are blended, releasing the organelles from the cell, then filtered to remove cell debris.

Question 7

what is the process by which homogenates are spun around

Answer 7

ultracentrifugation

Question 8

what is the order of the heaviest organelles

Answer 8

nucleus
mitochondria and chloroplasts
lysosomes
ER
ribosomes