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BotLab > Microscopy > Flashcards

Microscopy Flashcards

1
Q

A laboratory instrument used to examine objects that are too small to be seen by the naked eye

A

Microscope

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1
Q

Focuses light onto specimen using mirror or artificial light

A

Light Microscope

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2
Q

Example of light microscope that is used to view 3D objects and observing the surface of the specimen

A

Dissecting Microscope

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3
Q

Maximum Magnification of Dissecting Microscope

A

70x

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4
Q

Example of light microscope meant for looking through the specimen

A

Compound Microscope

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5
Q

Maximum Magnification of Compound Microscope

A

400x to 10000x

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6
Q

Uses beams of electrons to create an image

A

Electron Microscope

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7
Q

Have a much smaller wavelength than visible light that makes them produce higher resolution images

A

Electrons

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8
Q

Example of electron microscope designed for directly studying the surfaces of solid objects

A

Scanning Electron Microscope:

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9
Q

Example of electron microscope used to visualize the smallest structures in matter like cells in plants

A

Transmission Electron Microscope

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10
Q
  • Usually horse-shoe shaped
  • Supports the entire microscope
  • Provided with contact pads on the underside to give stable support on surfaces, balance, and stability when used in inclined position
A

Base

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11
Q

The slightly elevated part that connects base to the arm

A

Pillar

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12
Q

The curved part where one holds in carrying the microscope

A

Arm

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13
Q

A movable hinge that is used in tilting or inclining the microscope in a desired position

A

Inclination Joint

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14
Q

A cylindrical hollow tube attached to the arm and serves as a passageway of light from the primary and secondary magnifiers

A

Body Tube

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15
Q

A smaller cylinder at the upper part of the body tube that holds the eyepiece

A

Draw Tube

16
Q

A rotating head attached to the base of the body tube, which carries and facilitates the shifting of the objectives

A

Revolving Nosepiece

17
Q

A fixed circular disc above the nosepiece that protects the objective lens from dust

A

Dust Shield

18
Q

A square or rectangular plate with a flat surface on which the specimen rest

A

Stage

19
Q

A structure found below the stage that holds the condenser and iris diaphragm

A

Sub-stage

20
Q

A larger pair of knobs used when focusing the specimen with low power objective and lowering the body tube

A

Coarse Adjustment Knob

21
Q

A smaller pair of knobs used for final focusing of specimen with either low power or high power objectives

A

Fine Adjustment Knob

22
Q

It is an optical unit that is usually in the form of a detachable cylinder provided with lenses found above the draw tube

A

Eyepiece or Ocular

23
Q

It produces the magnified image of the object that shows all the detailed structure and general appearance

A

Objectives

24
Q

The shortest cylinder with large lens opening and has lower magnification. Used to obtain a large field of magnification, thus, observe the general view of the specimen

A

Low Power Objective (LPO)

25
Q

A longer cylinder with smaller lens opening than LPO. Used to obtain the detailed parts of the specimen with higher magnification

A

High Power Objective (HPO)

26
Q

The longest cylinder with a small opening. Used to observe fine details of specific parts with a very high magnification

A

Oil Immersion Objective

27
Q

Usually with a double-sided face with concave and plain surface; used when the light source is at low and very bright intensity, respectively

A

Mirror

28
Q

A simple optical unit that fills the field of view with uniform illumination and supplying the objective with a cone of light at the right size to produce maximum result

A

Condenser

29
Q
  • Attached at the base of the sub-stage and provided with a lever or projecting handle
  • Can be manipulated to regulate the amount of light passing through the lens
A

Iris Diaphragm

BotLab (5 decks)

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