Microscopy

Question 1

Answer 1

1. Ocular (eyepiece)
2. Body tube (eyepiece tube)
3. Obj ective
4. Stage
5. Aperture diaphragm and sub-stage condenser
6. Field diaphragm
7. Light source

Question 1

8. __ Controls the angle of light
9. __ Controls diameters of light rays
10. __ Focuses light onto specimen
11. __ Moves the specimen
12. __ Produces primary image
13. __ Produces secondary image
14. Provides illumination

A. Aperture diaphragm
B. Condenser
C. Field diaphragm
D. Light source
E. Mechanical stage
F. Ocular
G. Objective

Answer 1

8. A. Aperture diaphragm
9. C. Field diaphragm
10. B. Condenser
11. E. Mechanical stage
12. G. Objective
13. F.Ocular
14. D. Light source

Question 2

Place the following methods of improving contrast into their
appropriate category.
A. Supravital
B. Dry preparation

15. __ Acetic acid
    16.__ Gram stain
16. __ Hansel stain
    18.__ Oil Red 0
17. __ Sternheimer-Mal bin
18. __ Toluidine blue
19. __ Wright stain

Answer 2

15. A Acetic acid
    16.B Gram stain
16. B Hansel stain
    18.A Oil Red 0
17. A Sternheimer-Mal bin
18. A Toluidine blue
19. B Wright stain

Question 3

22. Which of the following lenses produces the primary image in brightfield microscopy?
    a. Condenser
    b. Iris
    c. Objective
    d. Ocular

Answer 3

c. Objective

Question 4

23. Which of the following types of microscopy uses fluorescence?
    a. Bright light
    b. Confocal
    c. Darkfield
    d. Interference

Answer 4

b. Confocal

Question 5

24. Which technique(s) would you use in order to determine if a urinary crystal demonstrates birefringence?
    a. Compensated light
    b. Darkfield
    c. Fluorescence
    d. Polarization

Answer 5

a. Compensated light
d. Polarization

Question 6

25. A specimen will appear three-dimensional when using
    which technique(s)?
    a. Differential contrast
    b. Modulation contrast
    c. Scanning electron microscopy
    d. Transmission electron microscopy

Answer 6

b. Modulation contrast
c. Scanning electron microscopy

Question 7

Place these steps in order for adjusting a microscope for Kohler
illumination.
26. __ Adjust the substage condenser.
27. __ Adjust the interpupillary distance.
28. Center the condenser.
29. __ Close field diaphragm.
30. __ Focus the specimen.
31. __ Open the field diaphragm.
32. __ Perform diopter adjustment.
33. __ Properly position sample.
34. __ Turn on microscope and adjust lamp intensity.
35. __ View the image with ocular removed and adjust
diaphragm; replace ocular.

Answer 7

26. 7 Adjust the substage condenser.
27. 3 Adjust the interpupillary distance.
28. 8 Center the condenser.
29. 6 Close field diaphragm.
30. 4 Focus the specimen.
31. 9 Open the field diaphragm.
32. 5 Perform diopter adjustment.
33. 2 Properly position sample.
34. 1 Turn on microscope and adjust lamp intensity.
35. 10 View the image with ocular removed and adjust diaphragm; replace ocular.