Chemical analysis of Urine

Question 1

Match the reagents listed below to the test in which they are used.
a. Bilirubin
b. Blood
c. Glucose
d. Ketone
e. Leukocytes
f. Nitrite
g. pH
h. Protein
i. Urobilinogen

1. _f. Nitrite __arsanilic acid
2. _g. pH __bromothymol blue
3. _b. Blood __cumene hydroperoxide
4. _a. Bilirubin __dichloroaniline
5. __i. Urobilinogen
   _dimethylaminobenzaldehyde
6. _e. Leukocytes __indoxylcarbonic acid ester
7. _g. pH __methyl red
8. __c. Glucose_potassium iodide
9. _d. Ketone __sodium nitroprusside
10. _h. Protein __tetrabromophenol blue
11. _h. Protein __tetrachlorophenol-tetrabromosulfophthalein
12. _f. Nitrite __tetrahydroquinoline
    1 3._b. Blood __tetramethylbenzine

Question 2

14. Specimens for urine chemistry analysis must be well
    mixed to ensure an accurate reading of:
    a. pH and specific gravity
    b. Blood and leukocytes
    c. Glucose and ketones
    d. Bilirubin and urobilinogen

Answer 2

b. Blood and leukocytes

Question 3

15. Timing of reagent strip readings is especially critical
    for:
    a. Diazo compound formation
    b. Dye-binding reactions
    c. Enzymatic reactions
    d. Protein error of indicators

Answer 3

c. Enzymatic reactions

Question 4

16. Testing specimens that contain high levels
    of ascorbate may affect the reading of all of these
    EXCEPT:
    a. Bilirubin
    b. Glucose
    c. Nitrite
    d. Urobilinogen

Answer 4

d. Urobilinogen

Question 5

17. A high specific gravity will affect all of the following
    reactions EXCEPT:
    a. Glucose
    b. Leukocytes
    c. Nitrite
    d. Protein

Answer 5

d. Protein

Question 6

18. Which of the following tests does not have a negative
    reading on reagent strip color charts?
    a. Blood
    b. Glucose
    c. Ketone
    d. Urobilinogen

Answer 6

d. Urobilinogen

Question 7

19. Purple colors are observed in the positive reactions for:
    a. Blood and glucose
    b. Ketone and leukocytes
    c. Bilirubin and urobilinogen
    d. Protein and nitrite

Answer 7

b. Ketone and leukocytes

Question 8

20. The ketone most detectable by all reagent strips is:
    a. Acetoacetic acid
    b. Acetone
    c. P-hydroxybutyric acid
    d. Phenylketone

Answer 8

a. Acetoacetic acid

Question 9

21. A false-positive protein may be produced by:
    a. Albumin
    b. Alkaline pH
    c. Ascorbic acid
    d. Run-over effect

Answer 9

b. Alkaline pH

Question 10

22. Positive bilirubin reactions should be
    confirmed by:
    a. Acetest
    b. Clinitest
    c. Foam Test
    d. Ictotest

Answer 10

d. Ictotest

Question 11

23. The principle of “protein error of indicators” is based on:
    a. Protein changing the pH of the specimen.
    b. Protein changing the pK. of the specimen.
    c. Protein accepting hydrogen from the indicator.
    d. Protein giving up hydrogen to the indicator

Answer 11

c. Protein accepting hydrogen from the indicator.

Question 12

24. Early detection of renal disease indicates testing for:
    a. Calcium
    b. Creatinine
    c. Macroalbumin
    d. Microalbumin

Answer 12

d. Microalbumin

Question 13

25. Non-routine urine reagent strip tests that may be useful
    to screen for substances associated with kidney stones
    include (choose all that apply).
    a. Ascorbate
    b. Calcium
    c. Creatinine
    d. Microalbumin

Answer 13

a. Ascorbate
b. Calcium