Microscopic Examination of Urine - Microscopy Flashcards
Most frequently used microscopy technique in the clinical laboratory
Bright field microscopy
Appearance of specimen when using bright-field microscope
Dark against a light background
The oldest and most common type of illumination system used on microscopes
Bright field microscopy
Type of microscopy in which variations in the specimen’s refractive index are converted into variations in light intensity or contrast
Phase contrast microscopy
Forms around the specimen when using phase contrast microscope
Halo formation
Microscopy technique that aids in identification of cholesterol in oval fat bodies, fatty casts, and crystals
Polarizing microscopy
Substance that rotates the plane in a clockwise direction has
Positive birefringence
Substance that rotates the plane in a counterclockwise direction has
Negative birefringence
Microscopy technique that aids in identification of spirochetes such as Treponema pallidum
Dark field microscopy
Appearance of specimen when using dark field microscope
Light against the black background
Microscopy technique that produces a three-dimensional microscopy-image and layer-by-layer imaging of a specimen
Interference contrast microscopy
Microscopy technique in which the difference in optical light paths through the specimen is converted into intensity differences in the specimen image
Interference contrast microscopy
Two types of interference-contrast microscopy
Modulation contrast (Hoffman)
Differential-interference contrast (Nomarski)
Microscopy technique that allows visualization of naturally fluorescent microorganisms or those stained by a fluorescent dye
Fluorescence microscopy
Lens system of a microscope
- Oculars
- Objectives
- Adjustment knobs
Illumination system of a microscope
- Light source
- Condenser
- Stage field
- Iris diaphragms
Body of a microscope
- Base
- Body tube
- Nose piece
Initial/Primary magnification of sample occurs in
Objectives
Final /Second magnification of sample occurs in
Eyepiece
Microscope component that regulates the angle of light presented to the specimen
Aperture diaphragm
The ability of a substance to refract light in two directions
Birefringent/ doubly refractile
Unequal refraction of light rays by a lens that occurs because the different wavelengths of light refract or bend at different angles
Chromatic aberration
Microscope component that gathers and focuses the illumination light onto the specimen for viewing
Condenser
The microscope lens or system of lenses located closest to the viewer’s eye
Eyepiece
