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CLINICAL MICROSCOPY > Microscopic Examination of Urine - Microscopy > Flashcards

Microscopic Examination of Urine - Microscopy Flashcards

1
Q

Most frequently used microscopy technique in the clinical laboratory

A

Bright field microscopy

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2
Q

Appearance of specimen when using bright-field microscope

A

Dark against a light background

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3
Q

The oldest and most common type of illumination system used on microscopes

A

Bright field microscopy

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4
Q

Type of microscopy in which variations in the specimen’s refractive index are converted into variations in light intensity or contrast

A

Phase contrast microscopy

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5
Q

Forms around the specimen when using phase contrast microscope

A

Halo formation

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6
Q

Microscopy technique that aids in identification of cholesterol in oval fat bodies, fatty casts, and crystals

A

Polarizing microscopy

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7
Q

Substance that rotates the plane in a clockwise direction has

A

Positive birefringence

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8
Q

Substance that rotates the plane in a counterclockwise direction has

A

Negative birefringence

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9
Q

Microscopy technique that aids in identification of spirochetes such as Treponema pallidum

A

Dark field microscopy

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10
Q

Appearance of specimen when using dark field microscope

A

Light against the black background

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11
Q

Microscopy technique that produces a three-dimensional microscopy-image and layer-by-layer imaging of a specimen

A

Interference contrast microscopy

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12
Q

Microscopy technique in which the difference in optical light paths through the specimen is converted into intensity differences in the specimen image

A

Interference contrast microscopy

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13
Q

Two types of interference-contrast microscopy

A

Modulation contrast (Hoffman)
Differential-interference contrast (Nomarski)

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14
Q

Microscopy technique that allows visualization of naturally fluorescent microorganisms or those stained by a fluorescent dye

A

Fluorescence microscopy

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15
Q

Lens system of a microscope

A
  1. Oculars
  2. Objectives
  3. Adjustment knobs
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16
Q

Illumination system of a microscope

A
  1. Light source
  2. Condenser
  3. Stage field
  4. Iris diaphragms
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17
Q

Body of a microscope

A
  1. Base
  2. Body tube
  3. Nose piece
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18
Q

Initial/Primary magnification of sample occurs in

A

Objectives

19
Q

Final /Second magnification of sample occurs in

A

Eyepiece

20
Q

Microscope component that regulates the angle of light presented to the specimen

A

Aperture diaphragm

21
Q

The ability of a substance to refract light in two directions

A

Birefringent/ doubly refractile

22
Q

Unequal refraction of light rays by a lens that occurs because the different wavelengths of light refract or bend at different angles

A

Chromatic aberration

23
Q

Microscope component that gathers and focuses the illumination light onto the specimen for viewing

A

Condenser

24
Q

The microscope lens or system of lenses located closest to the viewer’s eye

A

Eyepiece

25
Q

Microscope component that controls/regulates the diameter of light beams that strike the specimen and hence reduces stray light

A

Field diaphragm

26
Q

The circular field observed through a microscope

A

Field of view

27
Q

Type of microscopic illumination in which a lamp condenser focuses the image of the light source onto the front focal plane of the substage condenser

A

Köhler illumination

28
Q

Microscope component that holds the microscope slide with the specimen for viewing

A

Mechanical stage

29
Q

The lens or system of lenses located closest to the specimen

A

Objectives

30
Q

Term describing objective lenses that retain the same field of view when the user switches from one objective to another of a differing magnification

A

Parcenter

31
Q

Term describing objective lenses that remain in focus when the user switches from one objective to another of a differing magnification

A

Parfocal

32
Q

Ability of a lens to distinguish two points or objects as separate

A

Resolution

33
Q

A technique used to produce permanent microscope slides of urine sediment and body fluids

A

Cytocentrifugation

34
Q

Process of enlarging or magnifying an object’s size without affecting its actual or physical size

A

Magnification

35
Q

Magnification of scanner objective

A

4X

36
Q

Magnification of low power objective

A

10X

37
Q

Magnification of high power objective

A

40X

38
Q

Magnification of oil immersion field

A

100X

39
Q

Magnification of eyepiece

A

10X

40
Q

Total magnification of scanner objective

A

40X

41
Q

Total magnification of low power objective

A

100X

42
Q

Total magnification of high power objective

A

400X

43
Q

Total magnification of oil immersion objective

A

1000X

CLINICAL MICROSCOPY (20 decks)

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