Microscopes - Exam 2 Flashcards
light microscope
operate with visible light, use 2 magnifying lenses to achieve high magnification and clarity
compound microscope
magnify in stages using several lenses and can resolve structures that are separated by at least 200 nm
electron microscope
has 1000x the resolving power of a light microscope
transmission electron miscroscope
electrons used to visualize through material are capable of resolving objects only 0.2 nm apart (2x the diameter of H)
scanning electron microscope
beams electrons onto the surface of the specimen, electrons reflected back and electrons that it emits are amplified and transmitted onto a screen
immunohistochemistry
use of antibodies generated in animals that can be chemically bonded to the enzymes, stains, or fluorescent molecules after being purified