Microscopes Flashcards
What is a light microscope?
It uses light to magnify objects and its greatest possible magnification of x2000.
What is an electron microscope?
It uses electrons to view an object and it can magnify objects up to x10 million. You can see objects in cells more clearly and in far more detail than a light microscope.
How do you calculate magnification?
real size
1) measure image in mm.
2) multiply by 1000 to get the measurement in micrometres.
3) if the image has a scale bar the use that to find the answer.
How do you calculate magnification of an object?
magnification of eyepiece X
magnification of objective.
What are the smaller units?
milli- 10( power of -3 )
micro- 10( power of -6 )
nano- 10( power of -9 )
pico- 10( power of -12 )
How do you use a light microscope safely?
- start with the lowest power objective under the eyepiece.
- clip the slide securely on stage.
- adjust the light source ( mirror ) so the light goes through the slide.
- use the focusing wheel to focus on the slide.
- move the slide so its in the middle of the view.
- if you need move a higher power objective into position above the slide.
- use the focusing wheel to put the slide back into focus.