Microscopes Flashcards
What are the two types of focus in microscopes?
Coarse focus and fine focus
What is resolution in microscopy?
The minimum distance at which two points can be distinguished as separate
What does TEM stand for?
Transmission Electron Microscope
What is the maximum effective magnification of light microscopes?
About 1,000 times the actual size
What is contrast in microscopy?
The difference in brightness between light and dark areas of an image
What is the primary use of stereoscopic microscopes?
Viewing 3D objects like insects and leaves
How does fluorescence microscopy work?
Light of one wavelength is absorbed and light of a different wavelength is emitted
What range of light can human eyes see?
Between 400 and 600 nanometers
What is SEM used for?
Detailed study of specimen surface topography
What magnification is needed to see bacteria?
100x lens
What magnification is best for plant cells?
10x, 20x, or 40x
What’s unique about TEM images?
They provide 2D black and white views of internal cell structures
How is total magnification calculated?
By multiplying the power of all lenses used
What are fluorophores?
Fluorescent molecules used to label specific parts of cells
