Cell imaging and measurement Flashcards
What is flow cytometry?
A technique for making measurements on cells as they flow through a cytometer
What are biomolecules?
Organic molecules made of carbon, hydrogen, oxygen, nitrogen, sulfur, and phosphorus
What is hydrodynamic focusing?
Process of aligning cells in single file using a carrier fluid
What are the two types of measurements in flow cytometry?
Fluorescence and scatter
What is light scattering?
Physical process when light interacts with particles or boundaries between different mediums
What are the two main parts of flow cytometry?
Fluidics and optics
Why is calibration important in flow cytometry?
To ensure accuracy and precision of measurements
What does side scatter measure?
Internal complexity and granularity of cells
What are optical filters used for?
To allow selected wavelengths of light to pass while limiting others
Why is flow cytometry better than conventional cytometry?
It’s faster, less labor-intensive, and can measure more cells
What is bacteriophage?
A virus that targets and infects bacteria
What range can electron microscopes measure?
0.1 nm to 100 micrometers
What range can light microscopes measure?
100 nm to 1 cm
What is FACS?
Physical separation of cells with selected characteristics
How many cells can flow cytometry typically measure per second?
100+ cells, with some instruments measuring up to 10,000 cells
