Microscopes

Question 1

Draw and label a microscope.

Answer 1

Base
Mirror
Stage
Stage clip
Objective lens
Arm
Fine focus
Body tube
Nose piece
Coarse focus
Eyepiece

Question 2

What is the equation for magnification?

Answer 2

Magnification=image size/actual size

Question 3

EQ: describe how to use this microscope to observe a prepared slide.

Answer 3

1. Put the slide on the microscope stage.
2. Turn the nose piece to select the lowest power objective lens (this is usually ×4 objective lens).
   The end of the objective lens needs to almost touch the slide.
3. Turn the coarse adjustment knob to move the lens towards the slide. Look from the side (not
   through the eyepiece) when you are adjusting the lens.
4. Now look through the eyepiece. Slowly turn the coarse adjustment knob in the direction to
   increase the distance between the objective lens and the slide. Do this until the cells come into
   focus.
5. Slightly turn the fine adjustment knob to bring the cells into a clear focus. Use the low power
   objective lens (totalling 40 magnification) to look at the cells.
6. When you have found some cells, turn the nose piece to switch to a higher power lens (×100 or
   ×400 magnification).
7. You will have to use the fine adjustment knob again to bring the cells back into focus.

Question 4

Why do you start on a lower magnification?

Answer 4

So the FOV is wider increasing what you can see allowing you to find what you are looking for

Question 5

Why are microscopes important?

Answer 5

Because they allow us to learn and know about things we otherwise couldn’t know

Question 6

What are the 2 types of microscope?

Answer 6

Electron
Light

Question 7

Why would you use an electron microscope?

Answer 7

It has a greater resolution
It has a greater magnification