Microscope Part Functions Flashcards

1
Q

Eyepiece function

A

Holds ocular lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Arm

A

Supports tube and connects to base

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Revolving turret

A

Holds two or more objective lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Stage clip

A

Hold slide in place

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Stage

A

Platform to hold the slide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Condenser

A

Focuses light on the specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Micromanipulator

A

Moves stage along x and y axes

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Diaphragm

A

Controls amount of light passing through the specimen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Condenser adjustment knob

A

Adjusts focus of condenser

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Magnification of eyepiece lens

A

X10

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Coarse focusing knob

A

Bring specimen roughly into focal plane of objective lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Fine focusing knob

A

Make fine adjustments to achieve perfect focus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Steps of using microscope

A

Lower stage to lowest position
Lens clip opened, add slide, close the lens clip
Use micromanipulator to position specimen above condenser and below objective lens

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Resolution

A

How close 2 points in the image can be before they are no longer seen as 2 separate points

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What wavelength gives best resolution?

A

Shorter wavelength

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Contrast

A

Difference in lighting between adjacent areas of the specimen

17
Q

Diameter of an RBC

A

8 micrometres

18
Q

Focusing condenser lens on the specimen

A

Substage diaphragm fully open - change to x10 objective lens
Refocus specimen
Hold pencil point over the light source, move condenser lens away from stage - until point is in focus
Defocus slightly

19
Q

Adjusting substage iris diaphragm for optimum illumination

A

Withdraw one of eyepieces, stand upright
Close iris diaphragm, reopen so light fills 2/3 of field of view
Return eyepiece

20
Q

Difference between Olympus and Nikon microscopes

A

Differ by position of switch and illumination dial
Olympus - swinging eyepiece
Nikon - sliding eyepiece