Microscope Part Functions Flashcards
Eyepiece function
Holds ocular lens
Arm
Supports tube and connects to base
Revolving turret
Holds two or more objective lens
Stage clip
Hold slide in place
Stage
Platform to hold the slide
Condenser
Focuses light on the specimen
Micromanipulator
Moves stage along x and y axes
Diaphragm
Controls amount of light passing through the specimen
Condenser adjustment knob
Adjusts focus of condenser
Magnification of eyepiece lens
X10
Coarse focusing knob
Bring specimen roughly into focal plane of objective lens
Fine focusing knob
Make fine adjustments to achieve perfect focus
Steps of using microscope
Lower stage to lowest position
Lens clip opened, add slide, close the lens clip
Use micromanipulator to position specimen above condenser and below objective lens
Resolution
How close 2 points in the image can be before they are no longer seen as 2 separate points
What wavelength gives best resolution?
Shorter wavelength
Contrast
Difference in lighting between adjacent areas of the specimen
Diameter of an RBC
8 micrometres
Focusing condenser lens on the specimen
Substage diaphragm fully open - change to x10 objective lens
Refocus specimen
Hold pencil point over the light source, move condenser lens away from stage - until point is in focus
Defocus slightly
Adjusting substage iris diaphragm for optimum illumination
Withdraw one of eyepieces, stand upright
Close iris diaphragm, reopen so light fills 2/3 of field of view
Return eyepiece
Difference between Olympus and Nikon microscopes
Differ by position of switch and illumination dial
Olympus - swinging eyepiece
Nikon - sliding eyepiece
