Microscope & Cells F4 Flashcards
Function of the eyepiece lens
to look through
Objective lens magnification
total magnification is: eyepiece x objective lens
Function of the stage
holds slide and specimen
Function of the focusing knob
moves position of lens or stage to focus
Function of the light source
provides light to form an image
Why do you use the lowest power of objective lens when using a light microscope?
The field of view is wider, allowing to see more of the cells on the slide and making it easier to find the specimen
How do electron microscopes work?
They pass beams of electrons through a specimen
What does resolution mean?
The ability of a microscope to see in great detail
How do you find the true size of a biological specimen?
size observed / magnification
How do you find the magnification of an image of a biological specimen?
size observed / actual size
How do you find the magnification of an image?
measured length of scale line / given length of scale line
How do you prepare a slide of plant cells?
- take a clean slide
- lift small piece of tissue
- add drops of iodine solution
- view slide at low power
Why is iodine solution added?
To stain the cells and make them show clearly
Why does the piece of tissue need to be thin?
To allow light from the source to pass through the cells
How do you prepare a slide of animal cells?
- rub clean cotton bud on inside of cheek
- smear cotton bud on microscope slide
- add drop of methylene blue stain
- lower coverslip
- view on lowest power
What is the function of the vacuole?
contains cell sap and helps cell keep its shape
What is the function of the cell wall?
provides support to the cell
What is the function of the chloroplast?
contains chlorophyll to trap light during photosynthesis