Micropara finals Flashcards
Viruses
What cell type are viruses
Acellular
Viruses lack what?
Metabolic machinery
Identify the type of virus by its basic morphology:
Capsomers, Nucleic acid, Capsid
Naked virus
Identify the type of virus by its basic morphology:
Envelope, Capsid, Nucleic acid
Enveloped virus
Identify the DNA Viruses:
Parvovirus, Papovavirus, adenovirus, Iridovirus
Nonenveloped
Identify the DNA Viruses:
Hepadnavirus, Poxvirus, Herpesvirus
Enveloped
Identify the DNA Viruses:
Picornavirus, Reovirus
Nonenveloped
Identify the DNA Viruses:
Togavirus, Rhabdovirus, Orthomyxovirus, Bunyavirus, Coronavirus, Arenavirus, Retrovirus, Paramyxovirus
Enveloped al ss RNA
Enumerate the Family, Subfamily, Genus, and Species of Virus
Family - viridae
Subfamily - virinae
Genus - virus
Species - virus
What are the Primary characteristics of a virus?
Chemical nature, structure, Site of replication
What are the Secondary characteristics of a virus?
Host range, mode of transmission, Specific surface structures
Identify the characteristic of a virus:
RNA or DNA (ss or ds); + or - strand; MW
Chemical nature
Identify the characteristic of a virus:
shape, no of capsomeres, diameter of nucleocapsids
Structure of virion
Identify the characteristic of a virus:
Nucleus or cytoplasm
Site of replication
Identify the characteristic of a virus:
Host species; specific host tissues or cell types
Host range
Identify the characteristic of a virus:
e.g feces
Mode of transmission
Identify the characteristic of a virus:
e.g. antigenic properties
Specific surface structures
What icosahedral Viruses have No envelope
Papovaviridae, Picornaviridae
What Icosahedral viruses are ds DNA?
Herpesviridae, Hepadnaviridae
What icosahedral viruses have nucleus as their assembly site?
Papovaviridae, Herpesviridae, Hepadnaviridae
What are the similarities of all Helical viruses?
All have envelope and all have an assembly site of cytoplasm
Viruses that infect bacteria
Bacteriophages
Identify the type of bacteriophages:
Destroy their host cells (by lysis)
Lytic/Virulent
Identify the type of bacteriophages:
Do not destroy host cells
Integrate viral nucleic acid to host-cell genome and replicate without any cell lysis (Lysogeny)
Carried out in phages containing ds DNA
Temperate phages/ Avirulent
Enumerate the stages of virus replication
- Attachment (Adsorption)
- Penetration (injection)
- Synthesis of nucleic acid and protein 4
- Assembly and packaging
- Release (lysis)
Enumerate the steps of attachment of T4 bacteriophage virion to the cell wall of Escherichia coli and injection of DNA
(a) Unattached virion.
(b) Attachment to the
wall by the long tail
fibers interacting
with core
polysaccharide.
(c) Contact of cell wall
by the tail pins.
(d) Contraction of the
tail sheath and
injection of the DNA
True or False:
Unlike bacteriophages, animal viruses the entire virion is taken up into the cell
True
The Virus avoids the host’s immune response by
Growing inside cells
Viruses access to host cells because they have ______________________________ on host cells
attachment sites for host cells
Visible signs of viral infections are called
Cytopathic effects (CPE)
Is the smallest known infectious agents. Occurs only in plants, Does not posses protein coat
Viroids
Proteinaceous infectious particles. An unconventional virus
Prions
The possibility proteins are coded for by a gene found the normal host DNA
Altered protein
Medical-microbiology
Is a condition in which individuals of species live in close association with individuals of another species
Symbiosis
Identify the way in which symbiotic organisms interacts:
Form of symbiosis in which each organism benefit
e.g. bacteria in gut that aid digestion in both humans and animals and in turn obtain food from the human body
Mutualism
Identify the way in which symbiotic organisms interacts:
One organism benefits while the other organism neither benefits nor suffers from the interaction
e.g. this bacteria on epithelial surfaces such as in skin and respiratory tract
Commensalism
Identify the way in which symbiotic organisms interacts:
One organism lives in or on the body of another organism and is dependent on the host for its survival
e.g. viruses, parasitic worms, bacteria that causes strep throat, bubonic plague bacteria, cholera bacteria, etc.
Parasitism
Identify the way in which symbiotic organisms interacts:
The inhibition of one species of organisms by another
antibiotic production by microorganisms; myxobacteria and streptomycetes secreting lytic enzymes that degrade cell walls of other bacteria
Antagonism
Identify the way in which symbiotic organisms interacts:
As supply of nutrients decreases, rapidly growing species deprive those that grow slowly of food
e.g. microorganisms growing in fermented fruits/vegetables
Competition
Identify the way in which symbiotic organisms interacts:
One organism, the predator, feeds on and digests another organism, the prey
e.g. protozoa feed upon bacteria and some algae, bbdelovibrio bacteria feed upon other bacteria
Predation
Identify the characteristics of normal flora organisms:
due to chemical receptors in the body cell
e.g. proteins, polysaccharides, pili
Adhere to host cells
The detachment of host epithelial cells from body surfaces and replacement of the host cells by new cells
- affects microbial adherence
Desquamation
Identify the characteristics of normal flora organisms:
Residents flora produce metabolic products that can inhibit other microbes
Production of antimicrobial substances
This is considered the first line of defense
Skin
Inhibitory substances in the skin include
Sweat, lysozyme, sebum
The upper respiratory tract compromises of what?
Mouth, teeth, gum
Part of the Upper Respiratory Tract that is abundant in moisture, high dissolve food and food particles, saliva (10^8 bacteria per ml)
Mouth
Part of the Upper Respiratory Tract that is anaerobic environment
-G+anae = S. mutans
Teeth
Part of the Upper Respiratory Tract that is fac. anae (S. sanguinis) to anae (Actinomyces)
G- Porphyromonas (gingivitis);
motile Treponema denticola &
Trichomonas tenax (gingivitis)
Gum
Has no normal flora due to cilia-driven flow of mucus
Lower Respiratory Tract
True or False:
Note that the plaque builds preferentially near
the gum line, first occurring directly adjacent to the mucous membranes of the
gingiva.
True
Part of the Gastrointestinal Tract that has numerous transient
- fluid < 10 bacteria per ml (due to HCl and digestive enzymes)
- Lactobacilli, Candida sp.
Stomach
Part of the Gastrointestinal Tract that has a few strong acid and bile
Small intestine
Part of the Gastrointestinal Tract that is the largest population in the human body
- 10 ^11 orgs/g wt of stool (25% feces)
- 300 diff bacterial species from feces
- 3x 10^13 daily thru defecation
Large intestine
Part of the Genitourinary that is free of microorganisms
Kidneys, ureters, urinary bladder
Part of the Genitourinary
G+ = Staph. epidermidis,
Strep.faecalis,
Corynebacterium
G- cocci – Neisseria sp.
G- fac anae rods = E. coli
Lower urethra
Part of the Genitourinary
lactobacilli, enterococci,
Corynebacterium,
Candida albicans, high
anae bacteria
Vagina
A predominant organism in the vagina of women between the onset of puberty and the end of menopause.
Lactobacillus acidophilus
A pathogen that initiates response in healthy individuals
Primary pathogen
Causes infections that develop in people with low resistance, prolonged antibiotic therapy and immunosupressive therapy
Opportunistic pathogen
The degree of the ability of a pathogen to cause disease
Virulence
The microbial properties that enhance a microorganism’s pathogenicity
Virulence factors
Streptococcus pnuemoniae
w/ capsule = _______
w/o capsule = _______
w/ capsule = virulent
w/o capsule = avirulent
Identify the virulence factor:
capsules – resist phagocytosis
Cellular factors
Identify the virulence factor:
poisonous substances
Toxin producing
Toxin released in medium
Exotoxins
Toxin retained in cell
Endotoxins
Identify the virulence factor:
affects invasiveness (penetration of tissues)
Hyaluronidase produced by Strep. pyogenes in oral cavity
and Staph. aureus in skin – allows them to destroy
mucosa, affects tissue hydration & decelerates healing
Extracellular enzymes
Excreted by Gram + &
Gram – bacteria
Proteins
Heat labile
Convertible to toxoids
Specific for a particular
type of cell function
High toxicity ( minute
amounts)
Eg. C. botulinum toxin
Exotoxin
Released by lysed cells of
Gram – bacteria
Lipopolysaccharide
Heat stable
Cannot form toxoids
Generalized effect (eg.
shock & fever)
Low toxicity (larger
amounts)
E.coli toxin
Endotoxin
Factors Affecting Pathogenicity:
1. Cellular factors
2. Toxin producing
3. Extracellular enzymes
Properties inherent to the microbe
Factors Affecting Pathogenicity:
Resistance vs susceptibility
Ability of the host to resist infection
Steps for a pathogen to accomplish in order to cause disease
- infect the host
- metabolize & multiply
in host tissue - resist host defenses for
a time - damage the host
Type of resistance factor that is not directed at any one specific pathogen
Non-specific resistance factors
Type of resistance factors where host develops defense mechanisms in response to a specific pathogen, or specific resistance against that pathogen
Specific resistance factors
Internal defense mechanisms
A. Cellular mediators
B. Soluble mediators
C. Inflammation
D. Fever
External defense mechanisms:
chemical secretions
Lysozyme, sebum, lactoferrin, transferrin
Internal defense mechanism:
Cellular mediator that is non-phagocytic but kills tumor and virus-infected cells by binding to a target cell and releasing membrane-destroying protease and phospholipase enzymes
Natural killer cells
Internal defense mechanism:
Cellular mediator
Leukocytes (WBC)
Granulocytes – neutrophils, Eosinophils,
Basophils
Agranulocytes – lymphocytes, monocytes
Phagocytic cells
Internal defense mechanism:
Soluble mediator that 20 interacting proteins
(antimicrobial peptides) in serum – - activated by
invading mcgs or by antibody binding; some act as
protease, some act on surrounding inflammatory cells
-produced by mucous membrane cells &
phagocytes
Complement
Internal defense mechanism:
Soluble mediator that is soluble proteins produced and secreted by sensitized T lymphocytes
Lymphokines
Internal defense mechanism:
Soluble mediator are small proteins produced by eukaryotic cells in response to viral infection or foreign dsRNA
Interferons