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2A flash > micrococcacea > Flashcards

micrococcacea Flashcards

1
Q

family

A

micrococcaceae

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2
Q

genera

A

microccocus, staphylococcus, planococcis, stomacoccus (rothia)

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3
Q

free living saprophyte

A

planococcus

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4
Q

free living saprophyte, rarely cause human infection

A

micrococcus

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5
Q

normal flora of primates and other mammals

A

stomatococcus and staphylococcus

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6
Q

all, except ______ have been isolated from clinically significant sources

A

planococcus

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7
Q

these genus are always encountered in clinical sources or specimen

A

micrococcus and staphylococcus

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8
Q

can be isolated from clinically significant sources

A

microccocus
stomatococcus
staphylococcus

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9
Q

exists in the environment and doesn’t require or infect a host

A

planococcus

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10
Q

mostly associated with infections

A

staphylococcus

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11
Q

general characteristics (micrococcaceae)

A

-catalase positive, gram positive cocci
-resident flora of the skin and mucous membranes
-ubiquitous
-infections are directly or indirectly transmitted

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12
Q

differential test for micrococcus and staphlyococcus

A

Lysostaphin susceptibility test
Modified Oxidase test
Bacitracin susceptibility test
oxidation/fermentation (OF) test

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13
Q

incubation temp and time for Lysostaphin susceptibility test

A

35 - 37 deg C, 2 hours

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14
Q

antibiotic type of chemical that will destroy glycine rich peptide bond

A

lysostaphin

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15
Q

staphylococcus cannot grow properly in the presence of

A

lysostaphin

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16
Q

reagent use for modified oxidase test

A

Modified oxidase reagent:
6% tetramethyphenylenediamine hydrochloride in dimethyl sulfoxide
or
Microdase disk ( with impregnated Mod. oxidase reagent)

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17
Q

results in modified oxidase test can be observed

A

within 5-10 seconds

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18
Q

positive and negative result for Modified oxidase test

A

positive: micrococcus ( contains cytochrome oxidase enzyme)
negative: staphylococcus

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19
Q

test is used to differentiate micrococcus and stomatococcus from staphylococcus to 0.04 units of bacitracin

A

Bacitracin susceptibility test

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20
Q

_____ of bacitracin inhibits growth of ________ ,while having no effect on ______.

A

0.04 units, micrococcus/stomatococcus, staphylococcus

21
Q

zone of inhibition should be _____to indicate susceptibility and are typical of : micrococcus

A

greater than 10mm

22
Q

an organism that typically produce no zone of inhibition in bacitracin susceptibility test

A

staphylococcus

23
Q

testing if the organism can utilize the carbohydrate on certain environmental conditions

A

Oxidation/fermentation test

24
Q

uses two tubes of Hugh & leison’s medium

A

OF test

25
Q

in one tube of OF test the medium is ________

A

covered with vaseline

26
Q

both tube of OF test has what type of carbohydrate

A

glucose

27
Q

what dye is used for the ph indicator for OF test

A

bromthymol blue

28
Q

incubation temp and time duration

A

35 - 37 deg C and 18-24 hours

29
Q

alternate source of energy for asaccharolytic or non-fermentative organisms

A

peptone

30
Q

if the medium didnt turned yellow in a close system, what does it indicate

A

the organism can only utilize the carbohydrate in the presence of oxygen

31
Q

type of organisms that utilizes the carbohydrate in the presence of oxygen

A

oxidizers

32
Q

if the organism is capable of utilizing the carbohydrate with and without oxygen even in close tube

A

fermenter

33
Q

with or without oxgyen and no carbohydrate was utilize

A

nonutilizer

34
Q

will produce acid only in the open tube (without vaseline)

A

oxidizer (micrococcus)

35
Q

it will produce acid in both tubes

A

fermenter (staphylococcus)

36
Q

result if Micrococcus was OF tested, what color does it indicate and interpret

A

open: yellow
close: green

interpretation: oxidative; strictly aerobic

37
Q

result if Staphylococcus was OF tested, what color does it indicate and interpret

A

open: yellow
close: yellow

Interpretation: oxidative and fermentative; facultative anaerobic

38
Q

result of lysostaphin susceptibility test (200ug/ml)

A

susceptible: staphylococcus
resistant: micrococcus

39
Q

result of bacitracin susceptibility test

A

susceptible: micrococcus
resistant: staphylococcus

40
Q

result in furazolidone (100ug)

A

susceptible: staphylococcus
resistant: micrococcus

41
Q

result in lysosome (50-mg)

A

susceptibility: micrococcus
resistant: staphylococcus

42
Q

characteristic of Staphylococci

A

gram-positive cocci in clusters
catalase-positive

43
Q

morphology of staphylococci

A

spherical cell (0.5-1.5mm) that appear singly, in pairs, and clusters( depends on the plane of division of the organism)

44
Q

most common cause of food poisoning

A

S. aureus

45
Q

principal virulence factor of S. aureus

A

coagulated/staphylocoagulase

46
Q

can be cultivated by adding _____ to _____

A

7.5% to 10% NaCI

47
Q

Some strains produce golden yellow colonies due to the pigment they
produce known as ___________

A

Staphyloxanthin

48
Q

Most colonies beta-hemolytic (halo around the colonyent are presnt

A

beta-hemolytic

2A flash (11 decks)

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