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2A flash > histo technique (lab) > Flashcards

histo technique (lab) Flashcards

1
Q

preparation of tissue slices or “slices” that can be examined visually through transmitted light

A

Histotechnique

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2
Q

ideally, the tissue on the slide should have the same ________ as it had in the _____ .

A

structural features, body

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3
Q

accessioning: what are the requirement of the specimen to be accepted.

A

-suitable container
-covered or closed
-large enough to hold the specimen without distortion
-adequate amount of fixative (submerged fully)
-properly labeled
-correct requisition form

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4
Q

ratio of specimen to fixaive

A

1:20

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5
Q

type of examination that cuts a small section of a specimen and placing in a plastic cassettes.

A

Gross examination

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6
Q

who can perform the gross examination

A

Pathologist

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7
Q

tissue processing

A

-fixation
-dehydration
-clearing
-infiltration
-embedding
-sectioning
-deparaffinization
-staining
-mounting

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8
Q

tissue specimen is placed in solutions of chemicals that cross-link proteins and inactivate degradative cazymes

A

fixation

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9
Q

duration process of fixation

A

12-72 hours

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10
Q

type of fixatives

A

aldehydes:
-buffered isotonic 37% formaldehyde
-glutaraldehyde -electron microscopy
ALCOHOLS
*used for cytologic smears
-methanol (blood smear)
-ethanol (pap smear)

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11
Q

an optional tissue process if the specimen contains/has calcium

A

Decalcification

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12
Q

duration of the decalcification

A

days to weeks

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13
Q

decalcifying agents

A

Strong mineral acids
-nitric acids (most common)
-hydrochloric acid
weak organic acids
-formic acid
-trichloroacetic acid
Chelating agents
-EDTA

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14
Q

type of tissue process where water is extracted gradually by transfers through a series of increasing Ethanol solutions.

A

Dehydration

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15
Q

what is the concentration of the ethanol that the specimen should be dipped at

A

70%

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16
Q

what is the order of the ethanol concentration

A

70%,90%,100%

17
Q

type of process that give the tissue a translucent appearance

A

clearing

18
Q

type of clearing agents

A

-xylene
-toluene

19
Q

what is the most common clearing agent

A

xylene

20
Q

type of tissue process that place the specimen in a melted paraffin in an oven

A

Infiltration/Impregnation

21
Q

this step of Histotechnique evaporated the cleaning solvent

A

Infiltration/Impregnation

22
Q

type of automated processing machine

A

-tissue transfer machine/ rotary type
-fluid transfer machine/ vacuum type

23
Q

specimen is placed in a mold then filled with melted paraffin wax. what type of tissue processing?

A

Embedding

24
Q

this process ensure proper orientation of the specimen

A

embedding

25
Q

type of process that the block is placed in microtome for cutting

A

sectioning

26
Q

what should be the thickness of the specimen to view in a light microscopy

A

3-10 um

27
Q

what should be the thickness of the specimen to view in a electron microscopy

A

<1 um

28
Q

type of tissue process that removes the paraffin from the slide

A

deparaffinization

29
Q

type of tissue process that highlights the important features of the tissue as well as to enhance the tissue contrast.

A

staining

30
Q

anionic (negative charge)

A

basophilic

31
Q

cationic (positive charge)

A

acidophilic

32
Q

what is the most common used for tissue stain

A

Hematoxylin & Eosin (H&E)

33
Q

type of stain that highlights a tissue that contains glycogen & mucin

A

Periodic acid schiff (PAS) reaction

34
Q

type of stain that highlights tissue that has lipids

A

Sudan Black

35
Q

stain for silver and gold

A

metal impregnation

36
Q

type of tissue process the protects the specimen with glass coverslip with clear adhesive.

A

mounting

37
Q

Duration of fixatation if specimen is small

A

2-4hrs

38
Q
A

2A flash (11 decks)

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