Microbiology (workbook) Flashcards
Example of selective media?
MacConkey Agar
What is selective media?
Media that contains antibacterial substances which inhibit or kill all but a few types of bacteria
What is differential media?
Media that allows bacteria to be differentiated into groups by biochemical rxns on the media
Example of differential media?
Simmons citrate
What is enrichment media?
Liquid media that favor growth of a particular group of organisms; contains nutrients that encourage growth or desired organism or inhibitory substances that suppress competitors
Two examples of enrichment media
Tetrathionate broth, Selenite broth
What is transport media?
Media designed to keep microbes alive while not encouraging growth and reproduction
Example of transport media?
A culturette for specimen collection
Why do we heat fix prior to gram staining? (3)
Kills bacteria, fixes bacteria to slide, makes bacteria permeable to stain
4 methods of sample collection
Swabbing, scraping, aspiration, imprint
What type of agar is the base for all dermatophyte test media?
Sabouraud dextrose agar
A broth used for urine cultures
Thioglycollate broth
The catalase test is performed on (2)
Gram + cocci, small gram + bacilli
What is the reagent for the catalase test?
Hydrogen peroxide
Bacteria that prefer reduced O2 are called
Microaerophilic
Bacteria that require high levels of CO2 are called
Capnophilic
Bacteria come in 3 different shapes, they are?
Cocci, bacilli, spirochetes
Most bacteria thrive at body temp, which is? (In Celsius)
37 C
Fungal organisms consist largely of webs of slender tubes called
Hyphae
Partial hemolysis (green sludge) is called
Alpha hemolysis
Complete hemolysis is called
Beta hemolysis
Double zone hemolysis (both alpha and beta present) is called
Delta hemolysis
“Occult” hemolysis is called
Gamma hemolysis
The test used to aid bacterial classification when gram-variable results are obtained is the
KOH (potassium hydroxide) test
The stain used primarily to identify Mycobacterium and Nocardia species is
Ziehl-Neelsen (acid fast) stain
MIC stands for
Minimum Inhibitory Concentration
Hair infected with this organism may fluoresce a clear apple green under a Wood’s lamp
Ringworm
Micro organisms with complex nutritional requirements are referred to as
Fastidious
The lowest concentration of antimicrobial that will inhibit growth is the
MIC
