Microbiology (workbook)

Question 0

Example of selective media?

Answer 0

MacConkey Agar

Question 1

What is selective media?

Answer 1

Media that contains antibacterial substances which inhibit or kill all but a few types of bacteria

Question 2

What is differential media?

Answer 2

Media that allows bacteria to be differentiated into groups by biochemical rxns on the media

Question 3

Example of differential media?

Answer 3

Simmons citrate

Question 4

What is enrichment media?

Answer 4

Liquid media that favor growth of a particular group of organisms; contains nutrients that encourage growth or desired organism or inhibitory substances that suppress competitors

Question 5

Two examples of enrichment media

Answer 5

Tetrathionate broth, Selenite broth

Question 6

What is transport media?

Answer 6

Media designed to keep microbes alive while not encouraging growth and reproduction

Question 7

Example of transport media?

Answer 7

A culturette for specimen collection

Question 8

Why do we heat fix prior to gram staining? (3)

Answer 8

Kills bacteria, fixes bacteria to slide, makes bacteria permeable to stain

Question 9

4 methods of sample collection

Answer 9

Swabbing, scraping, aspiration, imprint

Question 10

What type of agar is the base for all dermatophyte test media?

Answer 10

Sabouraud dextrose agar

Question 11

A broth used for urine cultures

Answer 11

Thioglycollate broth

Question 12

The catalase test is performed on (2)

Answer 12

Gram + cocci, small gram + bacilli

Question 13

What is the reagent for the catalase test?

Answer 13

Hydrogen peroxide

Question 14

Bacteria that prefer reduced O2 are called

Answer 14

Microaerophilic

Question 15

Bacteria that require high levels of CO2 are called

Answer 15

Capnophilic

Question 16

Bacteria come in 3 different shapes, they are?

Answer 16

Cocci, bacilli, spirochetes

Question 17

Most bacteria thrive at body temp, which is? (In Celsius)

Answer 17

37 C

Question 18

Fungal organisms consist largely of webs of slender tubes called

Answer 18

Hyphae

Question 19

Partial hemolysis (green sludge) is called

Answer 19

Alpha hemolysis

Question 20

Complete hemolysis is called

Answer 20

Beta hemolysis

Question 21

Double zone hemolysis (both alpha and beta present) is called

Answer 21

Delta hemolysis

Question 22

“Occult” hemolysis is called

Answer 22

Gamma hemolysis

Question 23

The test used to aid bacterial classification when gram-variable results are obtained is the

Answer 23

KOH (potassium hydroxide) test

Question 24

The stain used primarily to identify Mycobacterium and Nocardia species is

Answer 24

Ziehl-Neelsen (acid fast) stain

Question 25

MIC stands for

Answer 25

Minimum Inhibitory Concentration

Question 26

Hair infected with this organism may fluoresce a clear apple green under a Wood’s lamp

Answer 26

Ringworm

Question 27

Micro organisms with complex nutritional requirements are referred to as

Answer 27

Fastidious

Question 28

The lowest concentration of antimicrobial that will inhibit growth is the

Answer 28

MIC