All Topics (notes) Flashcards
When examining the BUN/Creatinine ratio, if the BUN is disproportionately high, it could be due to (3)
Dehydration, dietary tx failure, owner non-compliance
How do you find the value of globulin?
Total protein - albumin
This test is used to rule out diabetes mellitus
Glucose tolerance
This test is used to determine the type of diabetes mellitus the animal has
Insulin tolerance
A pituitary function assay is
Growth hormone assay
Urine volume is controlled by what 2 hormones?
ADH; Aldosterone
This test is used to test for IMHA
Coomb’s test
How much blood do we collect for transfusion in dogs?
13-17 mL/kg
How much blood do we collect for transfusion in cats?
11-15 mL/kg
How much blood do we collect for transfusion in horses?
16 mL/kg
Mesocriles prefer what temperature?
20-40 C
Psycrophiles prefer what temperature?
Cold! At least below 20 F
Thermophiles prefer what temperature?
Hot!! At least above 40 C
What are the 4 bacterial growth phases?
Initial (lag), exponential, stationary, final (death)
What does a saprophytic fungi do?
Breaks down dead things and eats them
MacConkey agar is selective for
Lactose-fermenting gram - enterobacteria
Thioglycollate broth has areas for this type of bacteria to grow
Anaerobic
When using urea tubes, this indicates a bacteria that is positive
Pink color change
This test tests for motile bacteria
Sulfide indole motility
A positive result on a Simmons citrate looks like this
Blue color change
Triple sugar iron is media designed to grow
Salmonella
Brain heart infusion broth is media designed to
Increase numbers of bacteria
Mannitol salt agar is selective for
Staphylococcus
If staphylococcus aureus is present on mannitol salt agar, what will occur?
A yellow color change
Bismuth sulfate media is used for this organism
Salmonella
Mueller-Hinton agar is used for
Antimicrobial sensitivity testing
Sabouraud agar is used for this group of organisms
Dermatophytes
Microbiological samples sent to the lab need to be labeled with (11)
History, owner name, clinic name, clinic address/phone number, signalment of all animals affected or dead, duration of problem, major signs, ddx, organism suspected, tx given, investigation required
Parts of a gram stain
Crystal violet, iodine (mordant), alcohol (decolorizer), and saffranin
Bacterial colony characteristics (8)
Size, pigment, density, elevation, form, texture, odor, hemolysis
Bacterial colony forms can be described as (6)
Punctiform (little dots), circular, filamentous (Pom Pom), irregular, rhizoid (tree roots), spindle/lens (seed shaped)
Bacterial colony elevations can be described as (5)
Flat, raised, convex, pulvinate (tall and skinny), umbonate (boob)
Bacterial colony margins can be described as (6)
Even, undulate (wavy) filamentous (in needle like lines), lobate, erose (serrated), curled (in multiple lines like a bulls eye but wavy lines)
