Microbiology - Staphylococcus

Question 1

Staphylococcus Gram Stain

Answer 1

GPC in clusters

Question 2

Staphylococcus Colonies on SBA

Answer 2

smooth, Round

Question 3

Staphylococcus is seen as normal flora of the:

Answer 3

Skin, Mouth, Pharynx, Vagina, Urethra, GI tract

Question 4

Staphylococcus O2 requirements

Answer 4

Facultative anaerobe

Question 5

Staph spp. results
Catalase: ? Oxidase: ? Bactitracin: ? Furazolidone & Lysostaphine: ?

Answer 5

Staph spp. results
Catalase: POS Oxidase: NEG Bactitracin: R Furazolidone & Lysostaphine: S

Question 6

S. aureus pathogenicity (causes what?)

Answer 6

S. aureus causes suppurative cutanteous infections Toxic Shock Syndrome Food Poisoning

Question 7

S. aureus on SBA Beta hemolytic (small zone)

Answer 7

golden color

Question 8

S. aureus test results: Coagulase: ? Ferments: ? DNase & theronuclease: ?

Answer 8

S. aureus test results:
Coagulase: POS
Ferments: Mannitol (yellow)
DNase & theronuclease: POS

Question 9

Coagulase-Neg Staphylococci pathogenicity

Answer 9

Opportunistic (hospital acquired UTI)

Question 10

Coag neg staph on SBA

Answer 10

White, usually nonhemolytic

Question 11

50 - 80% of coag neg staph are:

Answer 11

S. epidermidis

Question 12

Staph saprophyticus pathogenicity (associated with)

Answer 12

UTIs in sexually active females
Urethritis
Prostatitis

Question 13

S. saprophyticus on SBA

Answer 13

White-yellow and NON hemolytic

Question 14

Micrococcus pathogenicity

Answer 14

Nonpathogenic
(found in environment, skin, mucous membranes, and oropharynx)

Question 15

Micrococcus on SBA Pigmented

Answer 15

(Bright yellow, orange, Pink, Tan)
High domed

Question 16

Micrococcus
Catalase: ?
Coagulase: ?
Oxidase: ?
Bactitracin: ?
Furazolindone & Lysostaphin: ?

Answer 16

Micrococcus
Catalase: POS
Coagulase: NEG
Oxidase: POS
Bactitracin: S
Furazolindone & Lysostaphin: R