Microbiology: Midterm Flashcards
Process that destroys or eliminates all forms of microbial life including endospores
Sterilization
The study of microorganisms
What is microbiology?
________ is known as the “Father of immuniology”
a) Louis Pasteur
b) John Tyndal
c) Joseph Lister
d) Sir Alexandar Flemming
e) Ignaz Philipp Semmelweis
a) Louis pasteur
Discovered the need for prolonged heating to destroy bacteria
a) Louis Pasteur
b) John Tyndal
c) Joseph Lister
d) Sir Alexandar Flemming
e) Ignaz Philipp Semmelweis
b) John Tyndal
Discovered Penicillin
a) Louis Pasteur
b) John Tyndal
c) Joseph Lister
d) Sir Alexandar Flemming
e) Ignaz Philipp Semmelweis
d) Sir Alexandar Flemming
Discovered the role of airborn microorganisms
a) Louis Pasteur
b) John Tyndal
c) Joseph Lister
d) Sir Alexandar Flemming
e) Ignaz Philipp Semmelweis
c) Joseph Lister
Recognized the importance of hand washing
a) Louis Pasteur
b) John Tyndal
c) Joseph Lister
d) Sir Alexandar Flemming
e) Ignaz Philipp Semmelweis
e) Ignaz Philipp Semmelweis
Portal of entry into the body
- Inhalation
- Ingestion
- Mucous Membranes
- Breaks in the skin
CDC
Centre for disease control
_______ collaborates to create the expertise, information, and tools that people and communities need to protect their health
CDC
Primitive cells
NO internal membrane
NO NUCLEUS
a) Prokaryotes cells
b) Eukaryote cells
a) Prokaryotes cells
Complex cells
With internal membranes.
HAVE A NUCLEUS
a) Prokaryotes cells
b) Eukaryote cells
b) Eukaryote cells
T/F: Bacteria is a Eukaryote cell
False.
Bacteria is a Prokaryotes cell
hair-like projections with fine short appendages that allow bacteria to adhere
Fimbriae
3 shapes of bacteria
- Cocci - Spherical
- Spirilla - Spiral
- Bacilli - Rod
Extreamly small; about one-tenth the size of bacteria
Obligate intracellular parasites
Viruses may be grouped into families but are not classified. They are defined by 3 things:
- Their type of nucleic acid: DNA or RNA
- The symmetry of the virus
- If they have an envelope
What is the sequence of Viral replication
- Attachment (Adsorbtion)
- Penetration
- Replication
- Assembly
- Release from host cell
One of the resistant forms of life
against heat, drying, and chemicals
Spores
Bacteria that develop a defense
mechanism against death
Spores
_______ grow best at body temperature
(37 degree C)
(with a range from 22-45 degrees C)
a) Psychrophiles
b) Thermophiles
c) Mesophiles
c) Mesophiles
______ grow best at warmer temperature (56 degree C)
(With a range of 45 – 70 degrees C)
a) Psychrophiles
b) Thermophiles
c) Mesophiles
b) Thermophiles
______ have optimal growth at refrigerator temperature
(7 degrees C)
(With range from 1- 22 degrees C)
a) Psychrophiles
b) Thermophiles
c) Mesophiles
a) Psychrophils
__________ have a pH 5.5-8.0
a) Neutrophiles
b) Alkalophiles pH
c) Acidophiles pH
a) Neutrophils
_______ have a pH 8.5-11.5
a) Neutrophiles
b) Alkalophiles
c) Acidophiles
b) Alkaphiles
_______ have a pH 1.0-5.5
a) Neutrophiles
b) Alkalophiles
c) Acidophiles
c) Acidophiles
What type of cell is this
a) Eukaryotic cell
b) Prokaryotic cell
a) Eukaryotic cell
What type of cell is this
a) Eukaryotic cell
b) Prokaryotic cell
b) Prokaryotic cell
_______ require oxygen at 20%
concentration
- Facultative anaerobes
- Microaerophiles
- Obligate Aerobes
- Obligate anaerobes
- Obligate Aerobes
tolerate low (4%) oxygen concentration
- Facultative anaerobes
- Microaerophiles
- Obligate Aerobes
- Obligate anaerobes
- Microaerophiles
cannot tolerate oxygen
- Facultative anaerobes
- Microaerophiles
- Obligate Aerobes
- Obligate anaerobes
- Obligate anaerobes
can grow with or without oxygen
- Facultative anaerobes
- Microaerophiles
- Obligate Aerobes
- Obligate anaerobes
- Facultative anaerobes
bacteria that produce acids during growth
a) Aciduric
b) Acidogenic
b) Acidogenic
bacteria that survive and grow in an acidic environment
(usually below pH 5.5)
a) Aciduric
b) Acidogenic
a) Aciduric
kill bacteria (chemical or physical)
a) Bacteriostatic agents
b) Bactericidal agents
c) Antibiotics
a) Bactericidal agents
prevent growth without killing them
a) Bacteriostatic agents
b) Bactericidal agents
c) Antibiotics
a) Bacteriostatic agents
To treat bacterial diseases
a) Bacteriostatic agents
b) Bactericidal agents
c) Antibiotics
c) Antibiotics
______ is a heat process employed to reduce # of bacteria and destroy pathogens
a) Bacteriostatic agents
b) Bactericidal agents
c) Pateurization
d) Lyphoilization
c) Pasteurization
a process of preserving microbes from a substance by removing the water, rapidly freezing the sample, then drying it by using a vacuum pump, at very low temperature (freeze drying)
a) Bacteriostatic agents
b) Bactericidal agents
c) Pateurization
d) Lyphoilization
d) Lyphoilization
For long term preservation of blood, serum, bacterial cultures, to preserve vaccines.
a) Bacteriostatic agents
b) Bactericidal agents
c) Pateurization
d) Lyphoilization
d) Lyphoilization
occurs when a microorganism in the body multiplies and causes damage to the tissue
a) Pathogen
b) Infectious disease
c) Opportunistic pathogens
d) Opportunistic pathogens
e) Exogenous diseases
f) Toxigenic diseases
b) Infectious disease
the microorganisms that cause infectious diseases
a) Pathogens
b) Infectious disease
c) Opportunistic pathogens
d) Endogenous diseases
e) Exogenous diseases
f) Toxigenic diseases
Pathogens
the causative agents of infectious diseases caused by microorganisms normally present or on or in the body, that will not cause harm unless favourably conditions exists for them to express their disease-producing potential
a) Pathogens
b) Infectious disease
c) Opportunistic pathogens
d) Endogenous diseases
e) Exogenous diseases
f) Toxigenic diseases
c) Opportunistic disease
are causes by microorganisms in or on the body
a) Pathogens
b) Infectious disease
c) Opportunistic pathogens
d) Endogenous diseases
e) Exogenous diseases
f) Toxigenic diseases
d) Endogenous disease
are caused by microorganisms not normally present on or in the body; will contaminate the body from the outside
a) Pathogens
b) Infectious disease
c) Opportunistic pathogens
d) Endogenous diseases
e) Exogenous diseases
f) Toxigenic diseases
e) Exogenous diseases
_________ microorganisms causing disease without entering & multiplying in the body (food poisoning)
a) Pathogens
b) Infectious disease
c) Opportunistic pathogens
d) Endogenous diseases
e) Exogenous diseases
f) Toxigenic diseases
f) Exogenous diseases
Viruses that infect bacteria
Bacteriophage
infection agent composed entirely of protein, causing a rare degenerative brain disease in humans called Creutzfeldt- Jacob
Prion
A method that prevents contamination by unwanted microorganisms - the absense of microbes
Asepsis
to consider all clients as being infected with bloodborne pathogens and therefore applying infection control
procedures to the care of all clients.
a) Standard precautions
b) Universal precautions
b) Universal precautions
A process that allows the transfer of microorganisms between persons and/or environmental surfaces or to a
sterile object. E.g. touching a clients chart with dirty gloves.
Cross-contamination
Are a set of infection control practices used to prevent the transmission of diseases through the contact with all body fluids (excepts sweat) including blood, non-intact skin & mucous membranes
a) Standard precations
b) Universal precation
Standard precautions
5 Pathways of cross-contamination
- Client to Dental Team
- Dental Team to Client
- Client to Client
- Dental Office to Community
- From Community to Client
Contact with microorganisms at the source as in the clients mouth. Touching soft tissue or teeth.
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Direct contact
Contact with items contaminated with a clients microorganisms
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Indirect contact
Contact with sprays, splashes, aerosols or splatter containing microorganisms.
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Droplet
smaller particles, can remain airborne for hours, can be inhaled
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Airborne
Tuberculosis, chickenpox, measles are examples of transmission by:
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Airborne
Influenza, mumps, rubella, herpes virus are examples of transmission by:
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Droplet
Hep B and C viruses are examples of transmission by:
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Indirect contact
Herpes infections of the fingers, STD’s, Skin diseases are examples of transmission by:
- Indirect contact
- Direct contact
- Airborne
- Droplet
- Direct contact
A process intended to destroy/kill
all mo’s in a material or on an object
a) Sterilization
b) Disinfection
a) Sterilization
A process that destroys mo’s, NOT
spores & viruses OR
a) Sterilization
b) Disinfection
b) Disinfection
The three common types of sterilization used in dentistry are:
- Heat
- Gas
- Liquid Chemical
A process that is intended to reduce and kill disease producing microorganisms but not bacterial endospores
Disinfection
6 types of disinfectants
- Alcohols
- Quaternary ammonium compounds
- Glutaraldehyde
- Iodophors
- Chlorine Compounds
- Phenols
sodium chloride loving bacteria
- Halophilic Bacteria
- Haloduric Bacteria
- Halophilic
capable of existing(do not prefer) in a
medium containing a high concentration of salt
- Halophilic Bacteria
- Haloduric Bacteria
- Haloduric
term used to designate all the categories of drugs used to treat
infections
Antibiotics
is meant to destroy or control a microbial infection to numbers that can
be eliminated by the immune system
Antibiotics
6 Antimicrobials
- Chlorhexidine (Peridex)
- Phenols (Listerine)
- Quaternary Ammonium Compound (Cepacol)
- Stannous Fluoride
- Sanguinarine (Viadent)
- Antibiotics
T/F: Antimicrobials are antibacterial
True
Antibiotics used in Dentistry
- Penicillin and its derivatives
- Tetracycline
- Erythromycin
A prolonged use of antimicrobials, especially an antibiotic can lead to:
- Toxicity
- Increase in incidence of allergic reactions
- Resistance to the antimicrobial
function as the powerhouse
Mitochondria
function to package protein; especially important for proteins destined for secretion
Golgi Apparatus
membranous sacs that contain digestive enzymes
Lysosomes
have cell walls composed of a thick layer
a) Gram positive
b) Gram negative
a) Gram positive
have cell walls with a thin layer
a) Gram positive
b) Gram negative
B) Gram negative
Bacteria cells divide by a process called
Binary Fission
Five major physical conditions influence growth of bacteria:
1.Temperature
2. Acidity
3. Nutrients
4. Oxygen metabolism
5. Water
enzymes released into the environment that break down proteins into amino acids that can enter the cell
Proteases
Common bacterial diseases
- TB
- Syphilis
- Anthrax
- Leprosy
- Pneumonia
- Dental Caries/Periodontal Disease
- Strep Throat
- Meningitis
Viral replication steps
- Attachment or adsorption
- Penetration
- Uncoating
- Replication or syntheses of virus
- Assembly
- Release from Host Cell
Ways of Transmission of Viruses
- Droplets
- Direct Transfer
- Contaminated food or water
- Insects