Microbiology: Generalities, Infection biology, Diagnostic microbiology, Parasitology Flashcards
Gram Staining Procedure
P = V M = I D = A C = S
Primary Stain = crystal Violet Mordant = Iodine Decolorizing Agent = Acetone Counterstain = Safranin
Gram Staining Procedure
Primary Stain = crystal Violet Mordant = Iodine Decolorizing Agent = Acetone Counterstain = Safranin
Bacteria NOT seen in Gram stain? Alternative approach?
These Rascals May Microscopically Lack Color
These Rascals May Microscopically Lack Color
Treponema - Spirochetes: Darkfield microscopy
Rickettsia - Giemsa/Tissue stains
Mycobacteria - Acid-Fast Stain
Mycoplasma - Serology
Legionella - Silver stain
Chlamydia - Giemsa stain (inclusion bodies)
Bacteria NOT seen in Gram stain? Alternative approach?
These Rascals May Microscopically Lack Color
Treponema - Spirochetes: Darkfield microscopy
Rickettsia - Giemsa/Tissue stains
Mycobacteria - Acid-Fast Stain
Mycoplasma - Serology
Legionella - Silver stain
Chlamydia - Giemsa stain (inclusion bodies)
Obligate Aerobes
Nosy and Nagging Pests Must Breathe Lots of oxygen
Nocardia Neisseria Pseudomonas Mycobacteria Bordetella/ Brucella/ Bacillus cereus Legionella
Obligate Aerobes
Nocardia Neisseria Pseudomonas Mycobacteria Bordetella/ Brucella/ Bacillus cereus Legionella
Obligate Anaerobes
ABC of Anaerobes
Actinomyces
Bacteroides
Clostridium
Obligate Anaerobes
Actinomyces
Bacteroides
Clostridium
Prophage Coded Bacteria
(Lysogenized strains) of ABCDE
(Lysogenized strains) of ABCDE: Non-essential, for virulence
shigA-like toxin: EHEC Botulinum Cholera Diphtheria Erythrogenic strain (of S. pyogenes/ GABHS/ scarlet fever)
Prophage Coded Bacteria
(Lysogenized strains) of ABCDE: Non-essential, for virulence
shigA-like toxin: EHEC Botulinum Cholera Diphtheria Erythrogenic strain (of S. pyogenes/ GABHS/ scarlet fever)
Normal Flora
Skin Nose Mouth Dental plaque Colon Vagina
Normal Flora
Skin: Staphylococcus epidermidis Nose: Staphylococcus aureus Mouth: Viridans streptococci Dental plaque: Streptococcus mutans Colon: Bacteroides, Escherichia coli Vagina: Lactobacillus vaginalis, Escherichia coli, Streptococcus agalactiae
Salma Hayek and her curli hair
Salmonella has surface proteins called ‘curli’ which mediate binding to endothelium and to extracellular proteins such as fibronectin
Enzyme in bacterial invasion, a.k.a. “spreading factor”
Hyaluronidase
What does IgA protease do?
Allows adherence to mucous membranes
Bacteria with IgA protease
SHine My Gong
Bacteria with IgA protease: SHiNe My Gong
Streptococcus pneumoniae
Haemophilus influenzae
Neiserria meningitidis
Neirserria gonorrhea
Streptococcus pyogenes anti-phagocytic factor
M protein
Staphylococcus aureus protein A function
Prevents complement activation
Which bacteria have “ADP-ribosylation” as the MOA of their exotoxins?
Diphtheria
Cholera
E.coli (heat-labile)
B. pertus
ADP-ribosylation is the addition of one or more ADP-ribose moieties to a protein. It is a reversible post-translational modification that is involved in many cellular processes, including cell signaling, DNA repair, gene regulation and apoptosis
Which bacteria have “superantigen” as the MOA of their exotoxins?
TSST (S. aureus)
Staphylococcal enterotoxin
Erythrogenic toxin (S. pyogenes)
Which bacteria have “protease” as the MOA of their exotoxins?
Tetanus (tetanospasmin, C. tetani)
C. botulinum
Lethal factor of B. anthracis toxin
Scalded skin toxin (S. aureus)
Which bacteria have “lecithinase” as the MOA of their exotoxins?
Clostridium perfringens alpha toxin
MOA of shiga toxin
Kills cells by cleaving 60S ribosomal subunit
Which bacteria have endotoxin?
eNdotoxin is an integral part of gram Negative cell walls
LPS toxic component: lipid A (overproduction of cytokines, complement cascade, coagulation cascade)
All bacteria have cell walls composed of peptidoglycan except
Mycoplasma pneumoniae