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CJJ > Microbiology > Flashcards

Microbiology Flashcards

1
Q

Gram Staining Sequence

A

Methyl Violet(Cystal Violet/Gentian Violet)
Grams Iodine(Mordant-Fixer)
Acetone(Decolonizer)
Safranin(Counterstain)

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2
Q

All Cocci are Gram Positive, except

A

Meningococci
Gonococci
Veilonella
Moraxella

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3
Q

All Bacilli are Gram Negative, except?

A 
Mycobacteria
Anthracis Bacillus 
Clostridium Species 
Diphtheriae Coryne Bacterium
Nocardia Species 
Acitomyces
Listeria 
Diphtheroids
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4
Q

ZN Staining
Other name?
Organisms?

A

Ziehl Neelson Staining/Acid Fast Staining

MTB 20%
M. Leprae 5%
Nocardia 1%

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5
Q

Ability to resist decolourisation comes because of____________content

A

Mycolic Acid Content

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6
Q

Sequence of ZN staining for MTB

A

For MTB Sputum Smear:

Sputum Smear 
Carbol Fuchsin
Intermittent Heating
20% H2SO4(Decolourizer)
Methylene Blue(Counter Stain)
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7
Q

Bipolar Staining Organisms

A 
Yersinia pestis(Plague)
Haemophilus ducreyi(Genital Ulcer)
Calymatobacter(Klebsiella)
Burkholderia Mallei(Glanders Disease-Horse Disease(motile))
Burkholderia Psuedo Mallei(motile)
Vibrio Parahemolytics(Sea food poisoning )
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8
Q

Cuneiform Arrangement is seen in________?

A

Albert Staining of Cornyebacterium Diphtheriae

Stained Green
AKA Chinese Letter Pattern

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9
Q

Capsulated Organisms

A 
Pneumococcus/Pseudomonas
Antarcis Bacillus 
Klebsiella
Influenza Haemophilus
Yersinia Species 
Bordetella Species 
Meningococci
Clostridium Perferingens(Welchii)
Vibrio Parahemolyticus
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10
Q

Most capsules are made of___________?

A

Polysaccharides

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11
Q

Capsules of Bacillus Anthracis is made of________?

A

Polypeptide

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12
Q

Quellung Reaction is seen in __________?

A

Pneumococcus

Capsular Antigen + Capsular Antibody = Capsular Swelling

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13
Q

MacFadyean’s Reaction is seen in ___________?

A

Bacillus Anthracis

Polypeptide capsule stained by Methylene Blue

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14
Q

Function of Capsule?

A

Antiphagocytic

Prevent Opsonisation

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15
Q

Opsonisers are?

Which is the major Opsoniser?

A
  1. C3b - (Major Opsoniser)

2. Fc of IgG and IgM

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16
Q

Function of Biofilm?

A
  1. Adherence to the surface
  2. Antibiotic Resistance

(Biofilm is modification of the capsule)

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17
Q

Tumbling Motility is seen in________?

A

Listeria

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18
Q

Darting motility is seen in____________?

A

Vibrio Cholera

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19
Q

Swarming Motility is seen in ____________?

A

Proteus

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20
Q

Falling Leaf like motility is seen in________?

A

Giardia Lamblia

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21
Q

Slow form of Swarming is called ________?

Its seen in ____________?

A

Stately Motility

Different species of Clostridium Species

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22
Q

Spirochetes are?

A

Endoflagella

arise from periplasmic space

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23
Q

Methods to demonstrate motility?

A
  1. Hanging Drop
  2. Semisolid Agar Method
  3. Phase Contrast Microscopy
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24
Q

Semi Solid Agar contains _______% agar

A

0.5-1% Agar

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25
Q

Nutrient Agar contains _____% agar

A

2% Agar

Contains peptone meat extract

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26
Q

Nutrient Broth contains _____% agar?

A

Zero % Agar

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27
Q

Enriched Media are?

A
  1. Blood Agar
  2. Chocolate Agar
  3. Loffler Serum Slope
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28
Q

Enriched media are solid/liquid?

A

Enriched - Solid

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29
Q

Blood Agar contains _______% blood?

A

5% Sheep Blood

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30
Q

Loffler Serum Slope is used for _______?

A

C. Diphtheriae

31
Q

Enrichment Media is Liquid/Solid?

A

Enrichment Media is always Liquid

32
Q

Enrichment media for Salmonella?

A

Selenite F Broth

33
Q

Enrichment media for Shigella?

A

Selenite F Broth

34
Q

Selective media is mostly liquid/solid?

A

Selective media is mostly solid?

35
Q

TCBS is ___________in colour?

TCBS is ___________ in colour when V. Cholera is used?

A

TCBS is Green in colour.

Turns to Yellow when V. Cholera is present.

36
Q

Selective Media for V. Cholera?

A 
TCBS
Thiosulphate 
Citrate
Bile Salt
Sucrose
37
Q

___________is the selective media for MTB?

A

Lowenstein Jensen(LJ) is the selective media for MTB

38
Q

_________ is the selective Agent for MTB in LJ Media?

A

Malachite Green is the selective Agent for MTB in LJ Media

39
Q

In MacConkey Agar, lactose fermenters show ___________ colour change?

A

In MacConkey Agar, lactose fermenters show PINK colour change

40
Q

In MacConkey Agar, non-lactose fermenters show ___________ colour change?

A

In MacConkey Agar, non-lactose fermenters show no colour change

41
Q

MacConkey Agar is a selective media for __________?

A

Only Gram Negative Bacteria will grow on MacConkey Agar

42
Q

Contents of MacConkey Agar?

A 
Peptone
Lactose
Agar
Neutral Red
Taurio Chocalate Sodium
43
Q

Lactose Fermenters are?

A

E coli
Klebsiella
Shigella Sonnei

44
Q

In Robertson’s Cooked Meat Media, when the meat pieces turn black, what is the reaction called?

A

When meat pieces turn black, it is a Proteolytic Reaction

45
Q

In Robertson’s Cooked Meat Media, when the meat pieces turn black, which is the organism present?

A

Clostridium tetani

Colour change due to Proteolytic reaction

46
Q

In Robertson’s Cooked Meat Media, when the meat pieces turn pink, which is the organism present?

A

Perferingens welchii

Sacchrolytic Reaction

47
Q

Transport media of V.Cholarae?

Which other Media can also be used?

A

V-R Media
Venkat Raman Ramakrishnan Media

Cary Blair Media can also be used

48
Q

Transport media of Streptococcus pyogenes?

49
Q

Transport media of Gonococeif meningococci?

A

Stuarts and Amies

50
Q

Transport media of Shigella(salmonella)?

A

Sach’s Buffered Glycerol Saline

51
Q

Obligate Anaerobes are?

A

Acitinomycetes
Bacteroids
Clostridium

52
Q

Facilitated Anaerobes are?

A

Staphylococcus

Streptococcus

53
Q

Capnophile organisms are?

A

Brucella abortus

Haemophilus influenza

54
Q

Micro aerophiles are?

A

Compylobacter

Helicobacter

55
Q

Organisms requiring -10 to +10°C is known as___________?

A

Psychrophile

56
Q

Organisms requiring 20 to 40°C is known as___________?

57
Q

Organisms requiring more than 45°C is known as___________?

A

Thermophile

58
Q

Organisms requiring more than 100°C is known as___________?

A

Thermus aquaticus

59
Q

Father of PCR?

A

Kary B Mullis

60
Q

____________ is Catalase Positive?

A

Staphylococcus is Catalase Positive

61
Q

____________ is Catalase Negative?

A

Streptococcus is Catalase Negative

62
Q

Coagulase Test Positive indicates __________?

Eg.____________

A

Pathogenic Staphylococcus is Coagulase Positive.

Staph. aureus

63
Q

Coagulase test Negative indicates______________?

Eg.___________

A

Non pathogenic Staphylococcus.
Eg.
Staph. Epidermis
Staph. Saprophyticus

64
Q

Staph. Epidermidis is Novobaicin ______?

A

Staph. Epidermidis is Novobaicin Sensitive

65
Q

Staph. Saprophyticus is Novobaicin_________?

A

Staph. Saprophyticus is Novobaicin Insensitive/Resistant

66
Q

Antibiotic Sensitivity Testing is conducted on _____________ agar?

A

AST is conducted on Mueller Hinton Agar(MHA)
(Nutrient Agar)

AST aka Kirby Baur Disc Diffusion Testing

67
Q

Antibiotic Sensitivity Testing is also known as?

A

Kirby Baur Disc Diffusion Testing

68
Q

Young women develops UTI.

MCC is?

A

MCC for female UTI is E.coli

69
Q

Female UTI

MC GPC is?

A

S.Saprophyticus

70
Q

Violet coloured grape like clusters are seen in __________ ?

A

Staph. aureus

71
Q

Staph. aureus in Nutrient Agar is seen as?

A

Golden Yellow Pigmentation

72
Q

Staph. aureus in Blood Agar is seen as?

A

B hemolysis

Pin head size colonies

73
Q

Best selective media for Staph. aureus?

A

Mannitol Salt Agar is the best

Ludham’s Media can also be used.

74
Q

Binding of Protein A (present in COWAN-1 strain of Staph. aureus) with FC portion of IgG is known as?

A

Co-agglution Test

CJJ (6 decks)

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