Biochemistry

Question 1

Which is the first irreversible step of Glycolysis?

Enzyme used?

Answer 1

G to G6PO4

Hexokinase
Glucokinase

Question 2

G6PO4 attaches to which all pathways?

Answer 2

Glycolysis
Glycogenesis
HMP Shunt

Question 3

Kinase transfers which group?

What is its cofactor?

Answer 3

Transfers PO4

Kinase uses Mg2+ as cofactor

Question 4

Glycolysis occurs in ________?

Answer 4

Cytosol

Question 5

Hexokinase is found in_____?

Answer 5

All tissues

Question 6

Glucokinase is found?

Answer 6

Adult liver and B pancreatic cells

Question 7

Hexokinase phosphorylate?

Answer 7

All Hexose Sugar

Glucose
Fructose
Galactose
Mannose

Question 8

Glucokinase phosphorylate ______?

Answer 8

Only Glucose

Question 9

Glucokinase activates only when BG is ______?

Answer 9

> 100mg/dl

Question 10

Km?

Answer 10

Susbstrate conc at which Velocity of reaction reaches 1/2 of Vmax

Question 11

Enzyme used to covert

G6PO4 to F6PO4

Answer 11

Isomerase

Pyranose to Furanose

Question 12

F6PO4 to F1, 6PO4

Enzyme used?

Answer 12

PFK 1

Phosphofructokinase 1

Question 13

Rate limiting enzyme of Glycolysis?

Answer 13

PFK 1

Question 14

Enzyme that converts big molecule to smaller molecules?

Answer 14

Lyase

Question 15

How much ATP does FADH2 and NADH2 give?

Answer 15

FADH2 1.5

NADH2 2.5

Question 16

FMN and FAD are actual forms of which Vitamin?

Answer 16

VitB2 (Riboflavin )

Question 17

NAD and NADP are active forms of ____?

Answer 17

VitB3 (Niacin)

Question 18

Which substance is used to vials clinically to inhibit Enalase?

Answer 18

NaF inhibits Enalase
Clinically it helps in correct estimation of BG levels

NaF and K+oxilate which act as Anticoagulant

Question 19

2nd MCC of Hemolytic Anemia?

Answer 19

Pyruvate Kinase Deficiency

Question 20

IRR of Glycolysis?

Answer 20

G to G6PO4(Hexo/Glucokinase)
F6PO4 to F1, 6PO4(PFK1)
PhosphoEnolPyruvate to Pyruvate(Pyruvate Kinase)

Question 21

Substrate level phosphorylation in Glycolysis?

Answer 21

1, 3BPG to 3 PG (PGK)

PEP to P(Pyruvate Kinase)

Question 22

End products of Aerobic Glycolysis?

Answer 22

2 Pyruvate + 7ATP

Question 23

End product of Anaerobic Glycolysis?

Answer 23

2 Lactate + 2 ATP