Biochemistry Flashcards
Which is the first irreversible step of Glycolysis?
Enzyme used?
G to G6PO4
Hexokinase
Glucokinase
G6PO4 attaches to which all pathways?
Glycolysis
Glycogenesis
HMP Shunt
Kinase transfers which group?
What is its cofactor?
Transfers PO4
Kinase uses Mg2+ as cofactor
Glycolysis occurs in ________?
Cytosol
Hexokinase is found in_____?
All tissues
Glucokinase is found?
Adult liver and B pancreatic cells
Hexokinase phosphorylate?
All Hexose Sugar
Glucose
Fructose
Galactose
Mannose
Glucokinase phosphorylate ______?
Only Glucose
Glucokinase activates only when BG is ______?
> 100mg/dl
Km?
Susbstrate conc at which Velocity of reaction reaches 1/2 of Vmax
Enzyme used to covert
G6PO4 to F6PO4
Isomerase
Pyranose to Furanose
F6PO4 to F1, 6PO4
Enzyme used?
PFK 1
Phosphofructokinase 1
Rate limiting enzyme of Glycolysis?
PFK 1
Enzyme that converts big molecule to smaller molecules?
Lyase
How much ATP does FADH2 and NADH2 give?
FADH2 1.5
NADH2 2.5
FMN and FAD are actual forms of which Vitamin?
VitB2 (Riboflavin )
NAD and NADP are active forms of ____?
VitB3 (Niacin)
Which substance is used to vials clinically to inhibit Enalase?
NaF inhibits Enalase
Clinically it helps in correct estimation of BG levels
NaF and K+oxilate which act as Anticoagulant
2nd MCC of Hemolytic Anemia?
Pyruvate Kinase Deficiency
IRR of Glycolysis?
G to G6PO4(Hexo/Glucokinase)
F6PO4 to F1, 6PO4(PFK1)
PhosphoEnolPyruvate to Pyruvate(Pyruvate Kinase)
Substrate level phosphorylation in Glycolysis?
1, 3BPG to 3 PG (PGK)
PEP to P(Pyruvate Kinase)
End products of Aerobic Glycolysis?
2 Pyruvate + 7ATP
End product of Anaerobic Glycolysis?
2 Lactate + 2 ATP
