Microbiology Flashcards
Eukaryote vs prokaryote ribosomes?
80S vs 70S
Prokaryote equivalent to the nucleus?
Nuceloid
Gram -ve summary?
- Stains pink
- Single-layered peptidoglycan wall
- Outer membrane
- High number of lipids, lipoproteins and lipopolysaccharides
- Produces endotoxins
Gram +ve summary?
- Stains purple
- Multi-layered peptidoglycan wall
- No outer cell wall
- Low/no lipids, lipoproteins and lipopolysaccharides
- Produces exotoxins
4 phases of bacterial growth?
Lag + exponential + stationary + death/decline
What is the peptidoglycan wall made of?
Sugars and amino acids
2 gram -ve cocci?
Neisseria meningitidis and gonorrhoeae
2 gram -ve coccibacilli?
H. influenza and B. pertussis
3 gram -ve bacilli?
Legionella, Klebsiella, Pseudomonas
Gram +ve bacilli?
Clostridium e.g. C. diff
Gram +ve cocci and test summary?
- Staph (+ve) and strep (-ve) via catalase test
- S. aureus (+ve) and s. epidermidis (-ve) via coagulase test
- Haemolysis for group A strep
- Alpha (green) = partial haemolysis via strep p. and viridans
- Beta (yellow) = full haemolysis via strep pyogenes
- Gamma (none) = staph
Gram +ve cocci cluster?
Staphylococcus
Gram +ve cocci chains?
Streptococcus
Selective media function + example?
Allows organisms to be isolated e.g. mannitol salt agar isolates staph
Differencial media function?
Chemicals produce visible changes in colonies via promoting differenciation
Bacteriostatic vs baceriocidal action?
Target protein/DNA synthesis which resume after removal vs target cell wall and cause cell death
Can you combine bacteriostatic/cidal abx?
No
Beta lactam abx?
Penicillins and cephalasporins