Microbiology Flashcards
Collect specimen in what phase?
acute phase of infection and before antibiotics
specimen is collected by
needle aspiration (tips of swab contain cotton, dacron, calcium alginate)
Blood culture (aerobic, anaerobic)
alcohol, iodine
adults: 20 mL per set
children: 5-10 mL per set
Body fluids collection
*sterile screw cap tube
*anaerobic transport sys
>1mL body fluids
>1mL bacterial culture
>1mL viral studies
>2mL fungal studies
>2mL AFB
Ear collection
Inner ear:
- clean w/ mild soap
- aspirate fluid w/ needle if eardrum is intact
Outer ear:
- remove debris w/ saline moist swab
- sterile tubes
Conjunctiva:
- sterile saline swabs
Corneal scrapings:
- scrape w/ sterile spatula
- inoculate into agar
Feces collection
- rectal swab
bacterial inf: 3 specimen, 3 days
parasitic inf: 3 specimen within 10 days
*collected before barium studies
Barium - white chalky substance, appearance of parasites
Genitalia collection
removes mucus
- swab vaginal mucosa
Urethra collection
insert 2-4 cm for 2-3 secs
Sputum collection
Myobacteria: 3 separate early morning specimens
Urine collection
clean catch midstream
- allow 15mL to pass
Specimen storage
4℃, 24 hrs
CSF: 35℃, 6 hrs
used to maintain accurate urine colony counts
Boric acid
Stools for clostridium difficile
collected without preservative, -70℃
most common anticoagulant used for microbiology specimens
sodium polyanethol sulfonate 0.025%
used for viral cultures and isolation of myobacterium spp. from blood
Heparin
commonly used in transport media
Stuart’s/Amie’s transport medium
contains selective agar, carbon dioxide generating tablet
JEMBEC system
growth of most nonfastidious microbes
nonselective media support
sheep blood agar
standard nonselective medium
