MICROBIO EXAM 3

Question 1

what is a genome

Answer 1

all the genetic elemeents present in an organism

Question 2

what is a chromosome

Answer 2

main geneetic element in the cell

Question 3

What is a plasmid

Answer 3

self replicating extrachromosomal genetic element

Question 4

geneotype

Answer 4

set of genes an organism has

Question 5

phenotype

Answer 5

effects or characteristics an organism displays

Question 6

function of sigma factor

Answer 6

recognize promoter region

Question 7

function of rho dependenet termination

Answer 7

rho attaches mRNA and influencing polymerase to fall offf

Question 8

what is factoir independent

Answer 8

this does not use rho and forms an inverted repeat of Urich region. it is weak and polymerase falls of

Question 9

Operon

Answer 9

genes ordered one after another with one promoter

Question 10

polycistrionic mRNA

Answer 10

open reading frame=coding region=genes

Question 11

constitutive genes

Answer 11

always expressed

Question 12

effect of sRNAs

Answer 12

antisense RNA
PREVENT or promote translation
prevent or promote degradation

Question 13

what is a global regulatory system

Answer 13

affects many genes and pathways at one time

Question 14

what is a mutation

Answer 14

heritage change in dna

Question 15

what is reversion mutation

Answer 15

mutation back to wildtype genotype

Question 16

suppresor mutation

Answer 16

mutation back to wild type genotype at a secondary site

Question 17

hfr strands

Answer 17

high frequency of recombinants

Question 18

DEscribe electrophoresis

Answer 18

it is an electric field across agarose gel. DNA is negatively charged and migrate towards positive electrode. small pieces migrate faster

Question 19

what is the importance of southern blot

Answer 19

uses nucleic acid probe to detect DNA fragment