MICROBIAL GROWTH AND NUTRITION MGN 1 Flashcards
Usual pH of microorganisms
pH 6.5-7.5
Why use white candle
Colored candles; dye - inhibitors
Species that require high salt amount
Vibrio
Vibrio pH
8-10
What is Lactobacillus acidophilus
Normal flora of vaginal tract
42 degree species (2)
Campylobacter jejuni, Pseudomonas aeruginosa
Absence of oxygen is needed
Strict or obligate anaerobe
Capnophilic requires what amount of CO2
5-10%
Thermophilic means
50-60 deg Celsius needed
Best time to take antibiotics
Logarithmic phase
Mesophilic temp
20-40 C
Stenothermophilic means
Short optimum temperature range
HACEK group is microaerophilic t or f, if false what should they be
False. Capnophilic
Needs cystine
Legionella pneumophila
major parts of bacterial metabolism
Anabolism, catabolism
Needs protein
Mycobacterium spp
24 hrs generation time
Mycobacterium tuberculosis
In death phase..
Culture becomes toxic to bacteria
Obligate/strict aerobe bacterias
Pseudomonas, Neisseria, Brucella, Bordetella, Francisella
3 metabolic pathways to breakdown glucose
EMP
PPP
EDP
This pathway is used by Brucella abortus and lactobacilli
Pentose Phosphate Pathway
This pathway converts glucose 6 phosphate to pyruvate
Entner Doudoroff Pathway
Direct microscopic count (2)
Petroff Hausser counter, Electronic cell counter
Escherichia coli generation time
20 minutes
2 basic bacteria groups
Autotrophs and Heterotrophs
Eurithermophilic means
Wide temp range
