Cell wall Flashcards
Thick murein layer
Gram (+)
Thin murein layer
Gram (-)
AF cell wall
Acid Fast
Hydrophobic
Acid fast
Acid fast is slightly ____?
Gram +. Weak
Three gram negative exceptions
Haemophillus influenzae
Neisseria gonorrhae
Bordetella pertussis
VIAS
Crystal violet, Iodine, Alcohol, Saffranin
PMDS
Primary stain, mordant, decolorizer, secondary stain
T or f. Mycolic acid is lipid rich
T
Acid fast means
Very difficult to remove
Acid fast staining CHAM
Carbol fuchsin, heat, acid, methylene blue or malachite green
T or f. Kinyoun is better than Ziehl Neelsen
F
Carbolic acid dissolves what
Fuchsin dye
for gram negative, the complex is removed due to
High lipid content
T or f crystal violet iodine complex wont retain in thick murein after decolorization
False
Gram positive has a thin peptidoglycan layer. T or f
Thick dapat. F
PMDS of Kinyoun method
Carbol fuchsin, tergitol, acid, meth blue
Filamentous organism. Gram p gram n or acid fast?
Acid fast
Classes of dyes
Basic and acid
Basic dyes are for
Cationic
Examples of basic dyes
Meth blue, basic fuchsin, crystal violet, saffranin
Examples of acid dyes
Rosin, rose bengal
Nigrosin and India ink r for
Negative staining
Stain used for specific parts of cell
Special stain
Spirochetes are apple green in color. T or f
Nope. Black
Fluorescent dyes are sensitive. T or f
True
