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BACTE (LEC) LESSON 1 > Microbial Growth And Nutrition > Flashcards

Microbial Growth And Nutrition Flashcards

1
Q

What are the 3 major nutritional needs for bacteria to grow.

A. Carbon, oxygen, Hydrogen
B. ATP, Carbon, Nitrogen
C. Nitrogen, Hydrogen, Oxygen
D. Carbon, Nitrogen, ATP

A

D

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2
Q

Source of ___ needs for making cellular constituents. It represents 50% of the dry weight of bacterium.

A

Carbon

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3
Q

___ is need for making proteins. It makes up 14% of the dry weight of bacterium.

A

Nitrogen

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4
Q

___ is need for performing cellular functions.

A

ATP

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5
Q

What is/are the 4% that makes up the weight of bacterium.

A

-phosphate (nucleic acid)
- phospholipid (cell membrane)
- sulfur (protein synthesis)

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6
Q

Metal and ions serve as ___ in bacterium.

A

Enzymes

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7
Q

Important mineral ions required by bacteria.

A

Na, K, Cl, Ca

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8
Q

Bacteria are classified based on their nutritional needs. What are they?

A

Autotrophs(lithotrophs)
Heterotrophs

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9
Q

Group of bacteria that are able to grow simply by CO2 as the sole source of carbon, with only water and inorganic salts.

A

Autotrophs

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10
Q

Autotrophs obtain energy either ____.

A
  • Photosynthetically (phototrophs)
  • inorganic compounds (chemolithotrophs)
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11
Q

Group of bacteria that requires more complex substances for growth.

A

Heterotrophs

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12
Q

They require organic source of carbon (glucose) and obtain energy by oxidizing or fermenting organic substance.

A

Heterotrophs

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13
Q

T or F
All bacteria that inhibit the human body fall into the autotropic group.

A

False- heterotrophic

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14
Q

T or F
E. coli and P. aeruginosa can use wide variety of organic compound.

A

True

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15
Q

T or F
H. influenzae and other aerobes require additional metabolites like vitamins.

A

False -anaerobes

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16
Q

A media whose contents are simple and completely defined.

A

Minimal media

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17
Q

T or F
Minimal media is the most common media used inside the lab.

A

False

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18
Q

Media that are more complex and made of extract soy bean and meat.

A

Nutrient media

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19
Q

Most common media

A

Nutrient media

20
Q

Nutrient medium contains ____.

A

Carbon and Nitrogen

21
Q

NB=
TSB=

A
  • Nutrient broth
  • Tryptic soy broth
    (Both colors are yellow)
22
Q

NA=
TSA

A

-Nutrient Agar
- Tryptic soy Agar

23
Q

Growth medium that contains added growth factors like blood, vitamins, and yeast is called what?

A

Enriched media

24
Q

Examples of enrich media
A. BAP, CHOC, CAP
B. MAC, BAP
C. CHOC, MAC
D. BAP, CHOC, CAP, MAC

25
Q

T or F
In BAP only gram positve grows in this media.

A

False- both gram positve and negative

26
Q

Media containing additives that only allow certain bacteria to grow.

A

Selective media

27
Q

Example of selective media

A
  • MacConkey agar (MAC) for gram-negative only
  • colistin-nalidixic acid (CNA) for gram-positive only
28
Q

Ingredients in media that allow visualization of metaboloc differences between groups of bacteria.

A

Differential media

29
Q

T or F
MAC can be both selective and differential media.

30
Q

If MAC was used as a diffential media, what are the two groups?

A
  • Lactose fermenter (pink)
  • Nonlactose fermenter (clear)
31
Q

T or F
BAP is only used for selective media

A

False- it can be differential or selective media

32
Q

If BAP was used as a differential media, what are the two classification?

A

Hemolytic and non hemolytic organisms.

33
Q

What are the 3 types of hemolysis in BAP media?

A

Alpha
Beta
Gamma

34
Q

Differentiate the characteristics of 3 types hemolysis in BAP.

A

Beta- complete hemolysis (yellow, clear)
Alpha- partial hemolysis (Greenish, Decolorization)
Gamma- no hemolysis (no changes)

35
Q

This media is used when there is a delay between the collection of specimens and culturing the specimens

A

Transport media

36
Q

This medium is designed to preserve the viability of microorganisms in specimen but doesn’t multiply.

A

Transport media

37
Q

Common examples of transport media.

A

Stuart broth
Amies
Cary-blair transport

38
Q

In broth media, what is the indication of turbidity.

A

The more turbid the broth, the more bacteria are present.

39
Q

T or F
BAP and MAC are usually used in lab.

40
Q

Which procedure is/are true about in preparing BAP?
A. NA sterilized in autoclave
B. After cooling down, add the blood
C. Most preferred blood is human blood
D. In human blood, type O is most prefered

41
Q

Preferred blood in BAP and CAP.

A

Horse or sheep blood (SBA)

42
Q

Inpreparing CHOC, how does the color of blood change into brown?

A

In preparing the CHOC, the bloood should be mixed in the NA while it is still hot. (70°C)

43
Q

CHOC/CAP=

A

Chocolate agar plate

44
Q

BAP=

A

Blood agar plate

45
Q

MAC=

A

MacConkey Agar plate

BACTE (LEC) LESSON 1 (9 decks)

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