Microbial Growth And Nutrition

Question 1

What are the 3 major nutritional needs for bacteria to grow.

A. Carbon, oxygen, Hydrogen
B. ATP, Carbon, Nitrogen
C. Nitrogen, Hydrogen, Oxygen
D. Carbon, Nitrogen, ATP

Answer 1

D

Question 2

Source of ___ needs for making cellular constituents. It represents 50% of the dry weight of bacterium.

Answer 2

Carbon

Question 3

___ is need for making proteins. It makes up 14% of the dry weight of bacterium.

Answer 3

Nitrogen

Question 4

___ is need for performing cellular functions.

Answer 4

ATP

Question 5

What is/are the 4% that makes up the weight of bacterium.

Answer 5

-phosphate (nucleic acid)
- phospholipid (cell membrane)
- sulfur (protein synthesis)

Question 6

Metal and ions serve as ___ in bacterium.

Answer 6

Enzymes

Question 7

Important mineral ions required by bacteria.

Answer 7

Na, K, Cl, Ca

Question 8

Bacteria are classified based on their nutritional needs. What are they?

Answer 8

Autotrophs(lithotrophs)
Heterotrophs

Question 9

Group of bacteria that are able to grow simply by CO2 as the sole source of carbon, with only water and inorganic salts.

Answer 9

Autotrophs

Question 10

Autotrophs obtain energy either ____.

Answer 10

• Photosynthetically (phototrophs)
• inorganic compounds (chemolithotrophs)

Question 11

Group of bacteria that requires more complex substances for growth.

Answer 11

Heterotrophs

Question 12

They require organic source of carbon (glucose) and obtain energy by oxidizing or fermenting organic substance.

Answer 12

Heterotrophs

Question 13

T or F
All bacteria that inhibit the human body fall into the autotropic group.

Answer 13

False- heterotrophic

Question 14

T or F
E. coli and P. aeruginosa can use wide variety of organic compound.

Answer 14

True

Question 15

T or F
H. influenzae and other aerobes require additional metabolites like vitamins.

Answer 15

False -anaerobes

Question 16

A media whose contents are simple and completely defined.

Answer 16

Minimal media

Question 17

T or F
Minimal media is the most common media used inside the lab.

Answer 17

False

Question 18

Media that are more complex and made of extract soy bean and meat.

Answer 18

Nutrient media

Question 19

Most common media

Answer 19

Nutrient media

Question 20

Nutrient medium contains ____.

Answer 20

Carbon and Nitrogen

Question 21

NB=
TSB=

Answer 21

• Nutrient broth
• Tryptic soy broth
  (Both colors are yellow)

Question 22

NA=
TSA

Answer 22

-Nutrient Agar
- Tryptic soy Agar

Question 23

Growth medium that contains added growth factors like blood, vitamins, and yeast is called what?

Answer 23

Enriched media

Question 24

Examples of enrich media
A. BAP, CHOC, CAP
B. MAC, BAP
C. CHOC, MAC
D. BAP, CHOC, CAP, MAC

Answer 24

A

Question 25

T or F In BAP only gram positve grows in this media.

Answer 25

False- both gram positve and negative

Question 26

Media containing additives that only allow certain bacteria to grow.

Answer 26

Selective media

Question 27

Example of selective media

Answer 27

- MacConkey agar (MAC) for gram-negative only - colistin-nalidixic acid (CNA) for gram-positive only

Question 28

Ingredients in media that allow visualization of metaboloc differences between groups of bacteria.

Answer 28

Differential media

Question 29

T or F MAC can be both selective and differential media.

Answer 29

True

Question 30

If MAC was used as a diffential media, what are the two groups?

Answer 30

- Lactose fermenter (pink) - Nonlactose fermenter (clear)

Question 31

T or F BAP is only used for selective media

Answer 31

False- it can be differential or selective media

Question 32

If BAP was used as a differential media, what are the two classification?

Answer 32

Hemolytic and non hemolytic organisms.

Question 33

What are the 3 types of hemolysis in BAP media?

Answer 33

Alpha Beta Gamma

Question 34

Differentiate the characteristics of 3 types hemolysis in BAP.

Answer 34

Beta- complete hemolysis (yellow, clear) Alpha- partial hemolysis (Greenish, Decolorization) Gamma- no hemolysis (no changes)

Question 35

This media is used when there is a delay between the collection of specimens and culturing the specimens

Answer 35

Transport media

Question 36

This medium is designed to preserve the viability of microorganisms in specimen but doesn't multiply.

Answer 36

Transport media

Question 37

Common examples of transport media.

Answer 37

Stuart broth Amies Cary-blair transport

Question 38

In broth media, what is the indication of turbidity.

Answer 38

The more turbid the broth, the more bacteria are present.

Question 39

T or F BAP and MAC are usually used in lab.

Answer 39

True

Question 40

Which procedure is/are true about in preparing BAP? A. NA sterilized in autoclave B. After cooling down, add the blood C. Most preferred blood is human blood D. In human blood, type O is most prefered

Answer 40

A,B,D

Question 41

Preferred blood in BAP and CAP.

Answer 41

Horse or sheep blood (SBA)

Question 42

Inpreparing CHOC, how does the color of blood change into brown?

Answer 42

In preparing the CHOC, the bloood should be mixed in the NA while it is still hot. (70°C)

Question 43

CHOC/CAP=

Answer 43

Chocolate agar plate

Question 44

BAP=

Answer 44

Blood agar plate

Question 45

MAC=

Answer 45

MacConkey Agar plate